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High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara

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Abstract

A protocol for in vitro propagation of Isodon wightii (Bentham) H. Hara from nodal segments was developed. Multiple shoots were successfully established on half strength MS medium supplemented with 4.4 μM BA. Enhancement of shoot multiplication and elongation was achieved on half strength MS medium supplemented with 4.4 μM BA and 1.4 μM GA3. The regenerated shoots were rooted successfully on half strength MS medium supplemented with 4.9 μM IBA. Acclimatization of in vitro rooted shoots was successful. The in vitro regenerated plants grew well in the greenhouse without any phenotypic changes.

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Fig. 1

Abbreviations

BA:

6-Benzyladenine

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

Kin:

Kinetin

NAA:

α-Naphthaleneacetic acid

TDZ:

Thidiazurone

Zea:

Zeatin

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Author notes

  1. R. Thirugnanasampandan

    Present address: Kongunadu Arts and Science College, GN Mills, Coimbatore, 641029, Tamil Nadu, India

Authors and Affiliations

  1. Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India

    R. Thirugnanasampandan, G. Mahendran & V. Narmatha Bai

Authors
  1. R. Thirugnanasampandan
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  2. G. Mahendran
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  3. V. Narmatha Bai
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Correspondence to R. Thirugnanasampandan.

Additional information

Communicated by J. Pauk.

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Thirugnanasampandan, R., Mahendran, G. & Narmatha Bai, V. High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara. Acta Physiol Plant 32, 405–409 (2010). https://doi.org/10.1007/s11738-009-0401-5

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  • DOI: https://doi.org/10.1007/s11738-009-0401-5

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