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Influence of nitric oxide in protection of tomato seedling against oxidative stress induced by osmotic stress

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Abstract

Osmotic stress associated with drought and salinity is a serious problem that inhibits the growth of plants mainly due to disturbance of the balance between production of ROS and antioxidant defense and causes oxidative stress. In this research, sodium nitroprusside (SNP) was used as NO donor in control and drought-stressed plants, and the role of NO in reduction of oxidative damages were investigated. In this study, we observed that SNP pretreatment prevented drought-induced decrease in RWC and membrane stability index, increase in lipid peroxidation and lipoxygenase activity and increase in hydrogen peroxide content. However, pretreatment of plants with SNP and phenyl 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (a NO scavenger) reversed the protective effects of SNP suggesting that protective effect by SNP is attributable to NO release. In addition, the relationship between these defense mechanisms and activity of antioxidant enzymes were checked. Results showed that in drought-stressed plants ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and catalase activities were elevated over the controls, while GR decreased under drought condition. Activity of GPX was inhibited under SNP pretreatment in drought-stressed plants specially, while the activity of APX and GR increased under SNP pretreatment and it seems that under this condition APX had a key role of detoxification of ROS in tomato plants. This result corresponded well with ASA and total acid-soluble thiols content. Therefore, reduction of drought-induced oxidative damages by NO in tomato leaves is most likely mediated through either NO ability to scavenge active oxygen species or stimulation of antioxidant enzyme such as APX.

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Authors and Affiliations

  1. Biology Department, Shahid Bahonar University of Kerman, Kerman, Iran

    Fatemeh Nasibi & Khosrow Manouchehri Kalantari

  2. International Center for Science, High Technology and Environmental Sciences, Kerman, Iran

    Fatemeh Nasibi

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  1. Fatemeh Nasibi
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  2. Khosrow Manouchehri Kalantari
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Correspondence to Khosrow Manouchehri Kalantari.

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Communicated by G. Bartosz.

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Nasibi, F., Kalantari, K.M. Influence of nitric oxide in protection of tomato seedling against oxidative stress induced by osmotic stress. Acta Physiol Plant 31, 1037–1044 (2009). https://doi.org/10.1007/s11738-009-0323-2

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  • DOI: https://doi.org/10.1007/s11738-009-0323-2

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