Abstract
Improving salt tolerance of economically important plants is imperative to cope with the increasing soil salinity in many parts of the world. Mutation breeding has been widely used to improve plant performance under salinity stress. In this study, we have mutagenized Echinochloa crusgalli L. with sodium azide and three selected mutants (designated fows A) with salt tolerant germination. Their vegetative growth was compared to that of the wild type after short-term and long-term salt stress. The germination of the three fows A mutants in the presence of inhibitory concentrations of NaCl, KCL, and mannitol was better than that of the wild type. Early growth of the mutants in the presence of 200 mM NaCl was also better than that of the wild type perhaps due to improved K+ uptake and enhanced accumulation of sugars particularly sucrose at least in two mutants. But the three mutants and the wild type responded similarly to long-term salt stress. The tolerance mechanisms during short-term and long-term salt stress are discussed.
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References
Apse MP, Aharon GS, Sneddon WA, Blumwald E (1999) Salt tolerance conferred by overexpression of a vacuolar Na1/H1 antiport in Arabidopsis. Science 285:1256–1258. doi:10.1126/science.285.5431.1256
Bates LE, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207. doi:10.1007/BF00018060
Bewley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066. doi:10.1105/tpc.9.7.1055
Bewley JD, Black M (1994) Seeds: physiology of development and germination. Plenum Press, New York
Blumwald E (2000) Sodium transport and salt tolerance in plants. Curr Opin Cell Biol 12:431–434. doi:10.1016/S0955-0674(00)00112-5
Blumwald E, Aharon GS, Apse MP (2000) Sodium transport in plant cells. Biochim Biophys Acta 1465:140–151. doi:10.1016/S0005-2736(00)00135-8
Boorman LA (1968) Some aspects of the reproductive biology of Limonium vlgare Mill. and Limonium humile Mill. Ann Bot (Lond) 32:803–824
Bradford KJ (1990) A water relation analysis of seed germination rates. Plant Physiol 94:840–849
Chandler PM, Marion-Poll A, Ellis M, Gubler F (2002) Mutants at the slender1 locus of barley cv Himalaya. Molecular and physiological characterization. Plant Physiol 129:181–190. doi:10.1104/pp.010917
Chen Z, Pottosin II, Cuin TA, Fuglsang AT, Tester M, Jha D, Zepeda-Jazo I, Zhou M, Palmgren MG, Newman IA, Shabala S (2007) Root plasma membrane transporters controlling K+/Na+ homeostasis in salt-stressed barley. Plant Physiol 145:1714–1725. doi:10.1104/pp.107.110262
Cuartero J, Fernandez-Munoz R (1999) Tomato and salinity. Scientia Hortic 78:83–125. doi:10.1016/S0304-4238(98)00191-5
Cuin TA, Shabala S (2006) Potassium homeostasis in salinised plant tissues. In: Volkov A (ed) Plant electrophysiology—theory and methods. Springer, Heidelberg, pp 287–317
de Castro RD, van Lammeren AAM, Groot SPC, Bino RJ, Hilhorst HWM (2000) Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not. Plant Physiol 122:327–335. doi:10.1104/pp.122.2.327
Dubcovsky J, Luo MC, Zhong GY, Bransteiter R, Desai A, Kilian A, Kleinhofs A, Dvorak J (1996) Genetic map of diploid wheat, Triticum monococcum L., and its comparison with maps of Hordeum vulgare L. Genetics 143:983–999
EA IA (1977) Manual on mutation breeding, 2nd edn. IAEA, Vienna
Epstein E, Norlyn JD, Rush DW, Kingsbury RW, Cunninham GA, Wrona AF (1980) Saline culture of corps: a genetic approach. Science 210:399–409. doi:10.1126/science.210.4468.399
Flowers TJ, Yeo AR (1995) Breeding for salinity resistance in crop plants: what next? Aust J Plant Physiol 22:875–884
Flowers TJ, Troke PF, Yeo AR (1977) The mechanism of salt tolerance in halophytes. Annu Rev Plant Physiol 28:89–121. doi:10.1146/annurev.pp.28.060177.000513
Gorham J, Bristol A, Young EM, Wyn Jones RG, Kashour G (1990) Salt tolerance in the Triticeae: K+/Na+ discrimination in barley. J Exp Bot 41:1095–1101. doi:10.1093/jxb/41.9.1095
Greenway H, Munns R (1980) Mechanisms of slat tolerance in non-halophytes. Annu Rev Plant Physiol 31:149–190. doi:10.1146/annurev.pp.31.060180.001053
Hasegawa PM, Bressan RA (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 51:463–499. doi:10.1146/annurev.arplant.51.1.463
Keiffer CH, Ungar IA (1997) The effect of extended exposure to hypersaline conditions on the germination of five inland halophyte species. Am J Bot 84:104–111. doi:10.2307/2445887
Khan MA, Ungar IA (1984) The effect of salinity and temperature on germination of polymorphic seeds and growth of Atriplex triangularis. Am J Bot 71:481–489. doi:10.2307/2443323
Ma JF, Tamai K, Ichii M, Wu GF (2002) A rice mutant defective in Si uptake. Plant Physiol 130:2111–2117. doi:10.1104/pp.010348
Maathuis FJM, Amtmann A (1999) K+ nutrition and Na+ toxicity: the basis of cellular K+/Na+ ratios. Ann Bot (Lond) 84:123–133. doi:10.1006/anbo.1999.0912
Macke AJ, Ungar IA (1971) The effect of salinity on germination and early growth of Puccinella nuttalliana. Can J Bot 49:515–520. doi:10.1139/b71-081
Molina-Cano JL, Simiand JP, Sopena A, Perez-Vendrell AM, Dorsch S, Rubiales D, Swanston JS, Jahoor A (2003) Mildew-resistant mutants induced in North American two and six-rowed malting barley cultivars. Theor Appl Genet 107:1278–1287. doi:10.1007/s00122-003-1362-5
Munns R (2005) Genes and salt tolerance: bringing them together. New Phytol 167:645–663. doi:10.1111/j.1469-8137.2005.01487.x
Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681. doi:10.1146/annurev.arplant.59.032607.092911
Olsen O, Wang X, Wettstein DV (1993) Sodium azide mutagenesis: Preferential generation of AT → GC transitions in the barley Antl8 gene. Proc Natl Acad Sci USA 90:8043–8047. doi:10.1073/pnas.90.17.8043
Peng Y-H, Zhu Y-F, Mao Y-Q, Wang S-M, Su W-A, Tang Z-C (2004) Alkali grass resists salt stress through high [K+] and an endodermis barrier to Na+. J Exp Bot 55:939–949. doi:10.1093/jxb/erh071
Qu X-X, Hung Z-Y, Baskin JM, Baskin CC (2008) Effect of temperature, light and salinity on seed germination and radicle growth of the geographically widespread halophyte shrub Halocnemum strobilaceum. Ann Bot (Lond) 101:293–299. doi:10.1093/aob/mcm047
Quesada V, Ponce MR, Micol JL (2000) Genetic analysis of salt-tolerant mutants in Arabidopsis thaliana. Genetics 154:421–436
Rubio F, Gassmann W, Schroeder JI (1995) Sodium-driven potassium uptake by the plant potassium transporter HKT1 and mutations conferring salt tolerance. Science 270:1660–1663. doi:10.1126/science.270.5242.1660
Shabala L, Cuin TA, Newman I, Shabala S (2005) Salinity-induced ion flux patterns from the excised roots of Arabidopsis sos1 mutants. Planta 222:1041–1050. doi:10.1007/s00425-005-0074-2
Shi H, Quintero FJ, Pardo JM, Zhu JK (2002) The putative plasma membrane Na+/H+ antiporter SOS1 controls long distance Na1 transport in plants. Plant Cell 14:465–477. doi:10.1105/tpc.010371
Stebbins GL (1971) Chromosomal evolution in higher plants. Arnold, London
Stitt M (1990) Fructose 2,6-bisphosphate. In: Lea PJ (ed) Methods in plant biochemistry. Academic Press, London, pp 87–92
Vicente MJ, Conesa E, Lvarez-Rogel JA, Franco JA, Martınez-Sanchez JJ (2007) Effects of various salts on the germination of three perennial salt marsh species. Aquat Bot 87:167–170. doi:10.1016/j.aquabot.2007.04.004
Werner JE, Finkelstein RR (1995) Arabidopsis mutants with reduced response to NaCl and osmotic stress. Physiol Plant 93:659–666. doi:10.1111/j.1399-3054.1995.tb05114.x
Zhang HX, Blumwald E (2001) Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol 19:765–768. doi:10.1038/90824
Zhu J-K (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273. doi:10.1146/annurev.arplant.53.091401.143329
Zwar JA (1995) α-Amylase production and leaf protein synthesis in a gibberellin-responsive dwarf mutant of “Himalaya” barley (Hordeum vulgare L.). Planta 197:39–48. doi:10.1007/BF00239937
Acknowledgment
We thank the Administration of Research (Mansoura University-Egypt) and the University of Sheffield for funding this work. We also thank Mr. Robert Coe for assistance with GC analysis of sugars.
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Communicated by F. Corbineau.
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Abogadallah, G.M., Quick, W.P. Vegetative salt tolerance of barnyard grass mutants selected for salt tolerant germination. Acta Physiol Plant 31, 815–824 (2009). https://doi.org/10.1007/s11738-009-0296-1
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DOI: https://doi.org/10.1007/s11738-009-0296-1