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Cu,Zn-superoxide dismutase is differently regulated by cadmium and lead in roots of soybean seedlings

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Abstract

The cadmium (Cd2+) and lead (Pb2+)-induced changes in Cu,Zn-SOD gene expression on the level of mRNA accumulation and enzyme activity were analyzed in roots of soybean (Glycine max) seedlings. The Cd2+ caused the induction of copper–zinc superoxide dismutase (Cu,Zn-SOD) mRNA accumulation, at each analyzed metal concentration (5–25 mg/l), whereas in Pb2+-treated roots this effect was observed only at the medium metal concentrations (50–100 mg/l of Pb2+). The analysis of Cu,Zn-SOD activity proved an increase in enzyme activity during Cd2+/Pb2+ stresses, however in Pb2+-treated plants the activity of enzyme was not correlated with respective mRNAs level. Presented data suggest that different metals may act on various level of Cu,Zn-SOD expression in plants exposed to heavy metals stress.

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  1. Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Ul. Umultowska 89, 61-614, Poznan, Poland

    Sylwia Pawlak, Anna Firych, Katarzyna Rymer & Joanna Deckert

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  1. Sylwia Pawlak
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  2. Anna Firych
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  3. Katarzyna Rymer
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  4. Joanna Deckert
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Correspondence to Joanna Deckert.

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Communicated by P. Wojtaszek.

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Pawlak, S., Firych, A., Rymer, K. et al. Cu,Zn-superoxide dismutase is differently regulated by cadmium and lead in roots of soybean seedlings. Acta Physiol Plant 31, 741–747 (2009). https://doi.org/10.1007/s11738-009-0286-3

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  • DOI: https://doi.org/10.1007/s11738-009-0286-3

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