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Enhancement of in vitro organogenetic capacity of rose by preculture of donor shoots on the medium with thidiazuron

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Abstract

Rose genotypes can be recalcitrant in in vitro regeneration. A lack of competence for regeneration results in unsatisfactory numbers of adventitious shoots, which diminishes achievements in use of genetic engineering in rose modern breeding. Among the factors which can partially overcome this problem is the use of thidiazuron (TDZ) in the induction phase of regeneration. We studied the use of TDZ in concentrations 0.05 and 1.0 mg l−1, in the medium for the culture of donor shoots with the aim of increasing the regeneration competence of five rose cultivars differing in their regeneration ability. All the tested genotypes increased their regeneration potential when the donor shoots were cultured on TDZ media for 15 or 30 days. The strongest effect, doubling to quadrupling the number of shoots, was obtained in the cultivars characterized by the lowest regeneration potential. This enhanced regeneration ability was maintained through two or three subsequent 4-week long regeneration passages.

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Authors and Affiliations

  1. Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100, Skierniewice, Poland

    D. Kucharska & T. Orlikowska

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  1. D. Kucharska
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  2. T. Orlikowska
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Correspondence to D. Kucharska.

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Communicated by E. Lojkowska.

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Kucharska, D., Orlikowska, T. Enhancement of in vitro organogenetic capacity of rose by preculture of donor shoots on the medium with thidiazuron. Acta Physiol Plant 31, 495–500 (2009). https://doi.org/10.1007/s11738-008-0258-z

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  • DOI: https://doi.org/10.1007/s11738-008-0258-z

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