Skip to main content

Advertisement

Springer Nature Link
Log in

In vitro clonal propagation of Clerodendrum serratum (Linn.) Moon (barangi): a rare and threatened medicinal plant

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

An in vitro process for rapid clonal propagation of Clerodendrum serratum (Linn.) Moon, a rare and threatened medicinal shrub, has been developed. Nodal stem segments having axillary bud, taken from field-grown plant, showed bud-break within 15 days of culture on modified Murashige and Skoog (MS) (Physiol Plant 15:473–497, 1962) medium supplemented with 0.25 mg/l each of 6-benzylaminopurine and indole-3-acetic acid along with 15 mg/l adenine sulphate (AdS). Regenerated shoots could be further multiplied on the same agarified morphogenetic medium in presence of 0.5 mg/l 2-chloroethyltrimethyl ammonium chloride with increased concentration of AdS, i.e., 30 mg/l. A group of five shoots used as inoculum produced on an average 4.98 new shoots per original shoot after 4 weeks of subculture. Shoots excised from cultures of proliferating shoots were rooted in half-strength MS medium having 1 mg/l indole-3-propionic acid. In vitro rooted shoots—plantlets—grew luxuriantly under field conditions and came to flowering after 10 months of transplantation. The genetic fidelity of in vitro-raised field-grown plants and their mother plant was ascertained by random amplified polymorphic DNA markers. The protocol developed holds good for in vitro cloning of C. serratum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

Abbreviations

AdS:

Adenine sulphate

BAP:

6-Benzylaminopurine

CCC:

2-Chloroethyltrimethyl ammonium chloride

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

IPA:

Indole-3-propionic acid

MS medium:

Murashige and Skoog (1962) medium

NAA:

α-Naphthaleneacetic acid

NOA:

α-Naphthoxyacetic acid

RAPD:

Random amplified polymorphic DNA

References

  • Chakrabarty D, Hahn EJ, Yun YS, Paek KY (2003) Micropropagation of apple root stock ‘M9 EMLA’ using bioreactor. J Hortic Sci Biotechnol 78:605–609

    CAS  Google Scholar 

  • Chaturvedi HC, Sharma AK, Agha BQ, Jain M, Sharma M (2004) Production of cloned trees of Populus deltoides through in vitro regeneration of shoots from leaf, stem and root explants and their field cultivation. Indian J Biotechnol 2:203–208

    Google Scholar 

  • Cheetam RD, Mikloiche C, Glubiak M, Weathers P (1992) Micropropagation of a recalcitrant male Asparagus clone (MD 22-8). Plant Cell Tissue Organ Cult 31:15–19

    Google Scholar 

  • CSIR (2001) The wealth of India: raw materials, vol 2. Publications and Information Directorate, CSIR, New Delhi, p 69

    Google Scholar 

  • Devi YS, Mukherjee BB, Gupta S (1994) Rapid cloning of elite teak (Tectona grandis Linn.) by in vitro multiple shoot production. Indian J Exp Biol 32:668–671

    CAS  Google Scholar 

  • Dewir YH, Chakrabarty D, Hahn EJ, Paek KY (2005) Reversion of inflorescence development in Euphorbia millii and its application to large-scale micropropagation in an air-lift bioreactor. J Hortic Sci Biotechnol 80:581–587

    CAS  Google Scholar 

  • Gupta SS, Gupta NK (1968) Development of antihistamine and anti-allergic activity after prolonged administration of a plant saponin from Clerodendrum serratum. J Pharm Pharmacol 20:801–802

    PubMed  CAS  Google Scholar 

  • Gupta SS, Rai M, Gupta NK (1967) Histamine releasing effects of a few Indian medicinal plants used in bronchial asthma. Curr Sci 36:42–43

    CAS  Google Scholar 

  • Juvekar AR, Nachankar RS, Hole RC, Wakade AS, Kulkarni MP, Ambaye RY (2006) In vitro and in vivo immunomodulatory activity of aqueous extract of Clerodendrum serratum L. roots. Planta Med 72. doi:10.1055/s-949887

  • Mao AA, Wetten A, Fay M, Caligari PDS (1995) In vitro propagation of Clerodendrum colebrookianum Walp, a potential natural anti-hypertension medicinal plant. Plant Cell Rep 14:493–496

    Article  CAS  Google Scholar 

  • Murashige T (1974) Plant propagation through tissue cultures. Plant Physiol 25:135–166

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Narayanan N, Thirugnanasambantham P, Viswanathan S, Vijayasekaran V, Sukumar E (1999) Antinociceptive, anti-inflammatory and antipyretic effects of ethanol extract of Clerodendrum serratum roots in experimental animal. J Ethnopharmacol 65:237–241

    Article  PubMed  CAS  Google Scholar 

  • Narayanan N, Thirugnanasambantham P, Viswanathan S, Rajarajan S, Sukumar E (2004) Comparative antibacterial activities of Clerodendrum serratum and Premna herbacea. Indian J Pharm Sci 66:453

    Google Scholar 

  • Rangaswami S, Sarangan S (1969) Sapogenins of Clerodendrum serratum constitution of a new pentacyclic triterpene acid, serratagenic acid. Tetrahedron 25:3701–3705

    Article  CAS  Google Scholar 

  • Rani V, Parida A, Raina SN (1995) Random Amplified Polymorphic DNA (RAPD) markers for genetic analysis of micropropagated plants of Populus deltoides Marsh. Plant Cell Rep 14:459–462

    Article  CAS  Google Scholar 

  • Rao PS, Bapat VA (1978) Vegetative propagation of sandalwood plants through tissue culture. Can J Bot 56:1153–1156

    Article  Google Scholar 

  • Rastogi RP (1999) Compendium of Indian medicinal plants, vol I, CDRI, Lucknow and NISC, New Delhi, p 113

  • Reuveni D, Shlecinger DR, Lavi U (1990) In vitro clonal propagation of dioecious Carica papaya. Plant Cell Tissue Organ Cult 20:41–46

    Article  Google Scholar 

  • Saghai-maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8028

    Article  PubMed  CAS  Google Scholar 

  • Sahoo Y, Chand PK (1998) Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high-frequency axillary shoot proliferation. Plant Cell Rep 18:301–307

    Article  CAS  Google Scholar 

  • Shanker Rai VR, Jagadish Chandra KS (1987) Clonal propagation of Cinnamomum zeylanicum Breyn. by tissue culture. Plant Cell Tissue Organ Cult 9:81–88

    Article  Google Scholar 

  • Shirin F, Rana PK, Mandal AK (2005) In vitro clonal propagation of mature Tectona grandis through axillary bud proliferation. J For Res 10:465–469

    Article  Google Scholar 

  • Ved DK, Kinhal GA, Ravikumar K, Karnat M, Vijaysankar R, Indresha JH (eds) (2003) Report of the threat assessment and management prioritisation (TAMP) for the medicinal plants of Chhattisgarh and Madhya Pradesh, TAMP Workshop, Bhopal, 23–26 July 2003

  • WWF India and ZOO/CBSG India (1997) North Indian Medicinal Plants categorized according to new IUCN Red List Criteria, under the Biodiversity Conservation Prioritization Project, by CAMP Workshop Process. U.P. Forest Department, Kukrail Park, Lucknow

    Google Scholar 

Download references

Acknowledgments

The authors express gratitude to The Director, National Botanical Research Institute, Lucknow, for the facilities provided and Botanic Garden Conservation International, UK, for partial financial support. Thanks are also due to Dr. R. L. S. Sikarwar, Senior Research Officer, Deendayal Research Institute, Chitrakoot, for providing the plant material.

Author information

Authors and Affiliations

  1. Tissue Culture Laboratory, National Botanical Research Institute, Lucknow, 226001, India

    M. Sharma, S. K. Rai, D. K. Purshottam, M. Jain, D. Chakrabarty, A. Awasthi & Ashok Kumar Sharma

  2. Conservation Biology and Molecular Taxonomy, National Botanical Research Institute, Lucknow, 226001, India

    K. N. Nair

Authors
  1. M. Sharma
    View author publications

    Search author on:PubMed Google Scholar

  2. S. K. Rai
    View author publications

    Search author on:PubMed Google Scholar

  3. D. K. Purshottam
    View author publications

    Search author on:PubMed Google Scholar

  4. M. Jain
    View author publications

    Search author on:PubMed Google Scholar

  5. D. Chakrabarty
    View author publications

    Search author on:PubMed Google Scholar

  6. A. Awasthi
    View author publications

    Search author on:PubMed Google Scholar

  7. K. N. Nair
    View author publications

    Search author on:PubMed Google Scholar

  8. Ashok Kumar Sharma
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Ashok Kumar Sharma.

Additional information

Communicated by E. Lojkowska.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sharma, M., Rai, S.K., Purshottam, D.K. et al. In vitro clonal propagation of Clerodendrum serratum (Linn.) Moon (barangi): a rare and threatened medicinal plant. Acta Physiol Plant 31, 379–383 (2009). https://doi.org/10.1007/s11738-008-0245-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-008-0245-4

Keywords