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Acta Physiologiae Plantarum

, Volume 30, Issue 3, pp 353–359 | Cite as

In vitro propagation of a multipurpose leguminous tree (Pterocarpus marsupium Roxb.) using nodal explants

  • Mohd Kashif Husain
  • Mohammad AnisEmail author
  • Anwar Shahzad
Original Paper

Abstract

Pterocarpus marsupium (Bijasal) is a valuable multipurpose forest tree. The regeneration rate in natural habitat is low and the tree is overexploited. An in vitro propagation protocol has been developed from nodal explants obtained from in vitro raised 18-day-old axenic seedlings. The highest shoot regeneration frequency (85%), maximum number of multiple shoots (8.6) as well as length (4.8 cm) were induced from nodal explants on Murashige and Skoog (MS) medium amended with 4.0 μM 6-benzyladenine (BA), 0.5 μM indole-3-acetic acid (IAA) and 20 μM adenine sulphate (AdS). The percentage of shoot multiplication as well as the number of shoots per node remained the same during the first two subculture, afterwards a decline was recorded. Rooting was best induced in microshoots excised from proliferated shoot cultures on semisolid hormone-free half-strength MS medium, after a pulse (dip) treatment for 7 days in half-strength MS liquid medium containing 100 μM indole-3-butyric acid (IBA) and 15.84 μM phloroglucinol (PG). The in vitro-raised plantlets were potted and acclimatized under culture room conditions for 4 weeks before their transfer to a greenhouse, where the established plants showed 75% survival.

Keywords

Leguminous tree Malabar kino Axillary bud break Multiple shoots In vitro plant regeneration 

Abbreviations

AdS

Adenine sulphate

BA

6-Benzyladenine

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

2iP

2-Isopentenyladenine

Kin

Kinetin

MS

Murashige and Skoog Medium

NAA

α-Naphthalene acetic acid

PG

Phloroglucinol (1,3,5-trihydroxybenzene)

Notes

Acknowledgments

The award of a Senior Research Fellowship to MK Husain by the Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi is greatly acknowledged. We also acknowledge the Department of Biotechnology (DBT), Government of India, New Delhi for financial assistance and The Director, Tropical Forest Research Institute (TFRI), Jabalpur, India in the procurement of seed material.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2008

Authors and Affiliations

  • Mohd Kashif Husain
    • 1
  • Mohammad Anis
    • 1
    Email author
  • Anwar Shahzad
    • 1
  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia

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