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Micropropagation of gerbera: lipid peroxidation and antioxidant enzyme activities during acclimatization process

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Abstract

Gerbera jamesonii H. Bolus ex Hook (Family: Asteraceae) has been successfully acclimatized from temperate to subtropical North Indian plains of Lucknow through in vitro propagation. Flower heads were collected from greenhouse, segmented into 4–16 pieces and cultured in Murashige and Skoog’s medium (MS) (Physiol Plant 15:472–497, 1962) supplemented with 2.87 μM indole-3-acetic acid (IAA) and 8.88 μM N6-benzyladenine (BA) for shoot regeneration. Shoots were subcultured on growth regulator free MS medium. Apical shoot meristems from in vitro plantlets of gerbera were tested in MS medium with different combination of cytokinins [BA, kinetin, and thidiazuron (TDZ)] alongwith 2.68 μM 1-naphthaleneacetic acid (NAA) for shoot multiplication. The optimum results were obtained with 8.88 μM BA. Regenerated plants with well-established root system were transferred to pots containing soil and sand (1:1 v/v) and were kept in humidity chamber with 80–90% relative humidity for 0, 5, 10, 15, 20, and 25 days before they were transferred to field (during October, 2005 to February, 2006). Survival percentage was higher when regenerated plantlets were kept under humidity chamber for 15 days. An attempt was made to obtain basic information on different biochemical changes during acclimatization process of in vitro raised plantlets. Increased lipid peroxidation and high H2O2 content in early stages of acclimatization process reflected a similar process of oxidative stress. Our work suggests that tissue-cultured plants develop antioxidant enzymatic protective system which determine the ability to survive in oxidative stress and up regulation of these enzymes would help to reduce the built up of reactive oxygen species (ROS).

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Abbreviations

AsA:

Ascorbate

APX:

Ascorbate peroxidase

BA:

N6-benzyladenine

CAT:

Catalase

EDTA:

Ethylenediamine tetraacetic acid

IAA:

Indole-3-acetic acid

GR:

Glutathione reductase

KI:

Potassium iodide

MDA:

Malondialdehyde

MDHA:

Monodehydroascorbate

MS:

Murashige and Skoog’s medium

NAA:

1-naphthaleneacetic acid

NBT:

Nitro blue tetrazolium

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TDZ:

Thidiazuron

TBA:

Thiobarbituric acid

TCA:

Trichloracetic acid

References

  • Aebi H (1974) Catalases. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 2. Academic, New York, pp 673–684

  • Ahmed S, Nawata E, Hosokawa M, Domae Y, Sakuratani T (2002) Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Sci 163:117–123

    Article  CAS  Google Scholar 

  • Alexieva V, Sergiev I, Mapelli S, Karanov E (2001) The effect of drought and UV on growth and stress markers in pea and wheat. Plant Cell Environ 24:1337–1344

    Article  CAS  Google Scholar 

  • Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases in controlling oxidative stress in plants. J Exp Bot 53:1331–1341

    Article  PubMed  CAS  Google Scholar 

  • Bailly C, Benamar A, Corbineau F, Dôme D (1996) Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seed as related to deterioration during accelerated aging. Physiol Plant 97:104–110

    Article  CAS  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  PubMed  CAS  Google Scholar 

  • Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in condition. Anal Biochem 161:559–566

    Article  PubMed  CAS  Google Scholar 

  • Bowler CM, van Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43:83–116

    Article  CAS  Google Scholar 

  • Bowler C, Van Camp W, Van Montagu M, Inze D (1994) Superoxide dismutase in plants. CRC Crit Rev Plant Sci 13:199–218

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Chen GX, Asada K (1989) Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol 30:987–998

    CAS  Google Scholar 

  • Datta SK, Mitra R (1999) Commercial floriculture in India. In: Applied Botany Abstracts, IBIS, NBRI, vol 19, pp 202–221

  • Dewir YH, Chakrabarty D, Hahn EJ, Paek KY (2006) A simple method for mass propagation of spathiphyllum using an air-lift bioreactor. In Vitro Cell Dev Plant 42:291–297

    Article  CAS  Google Scholar 

  • Gillham DJ, Dodge AD (1986) Hydrogen peroxide scavenging systems in pea chloroplasts. Planta 167:246–251

    Article  CAS  Google Scholar 

  • Hazarika BN (2003) Acclimatization of tissue-cultured plants. Curr Sci 85:1704–1712

    CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  PubMed  CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  • Laliberte S, Chretien L, Vieth J (1985) In vitro plantlet production from young capitulum explants of Gerbera jamesonii. HortScience 20:137–139

    Google Scholar 

  • Larson RA (1988) The antioxidants of higher plants. Phytochemistry 27:969–978

    Article  CAS  Google Scholar 

  • Misra P, Datta SK (2001) Acclimatization of Asiatic hybrid lilies under stress conditions after propagation through tissue culture. Curr Sci 81:1530–1533

    Google Scholar 

  • Mittler R, Zilinskas BA (1993) Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate dependent reduction of nitroblue tetrazolium. Anal Biochem 212:540–546

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279

    Article  PubMed  CAS  Google Scholar 

  • Noctor G, De Paepe R, Foyer CH (2007) Mitochondrial redox biology and homeostasis in plants. Trends Plant Sci 12:125–134

    Article  PubMed  CAS  Google Scholar 

  • Orlikowska T, Nowak E, Marasek A, Kucharska D (1999) Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from gerbera petioles. Plant Cell Tissue Organ Cult 59:95–102

    Article  CAS  Google Scholar 

  • Pierik RLM, Jansen JLM, Maasdam A, Binnendijk CM (1975) Optimalization of Gerbera plantlet production from excised capitulum explants. Sci Hortic 3:351–357

    Article  Google Scholar 

  • Sairam RK, Deshmukh PS, Saxena DC (1998) Role of antioxidant systems in wheat genotypes tolerance to water stress. Biol Plant 41:387–394

    Article  CAS  Google Scholar 

  • Sairam RK, Deshmukh PS, Shukla DS (1997) Tolerance to drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. J Agron Crop Sci 178:171–177

    Article  CAS  Google Scholar 

  • Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis (2-nitrobenzoic acid). Anal Biochem 175:408–413

    Article  PubMed  CAS  Google Scholar 

  • Van Huylenbroeck JM, Piqueras A, Debergh PC (2000) The evolution of photosynthetic capacity and the antioxidant enzymatic system during acclimatization of micropropagated Calathea plants. Plant Sci 155:59–66

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Floriculture Section, National Botanical Research Institute, Lucknow, Uttar Pradesh, India

    Debasis Chakrabarty & Subodh Kumar Datta

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  1. Debasis Chakrabarty
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  2. Subodh Kumar Datta
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Correspondence to Debasis Chakrabarty.

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Communicated by G. Bartosz.

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Chakrabarty, D., Datta, S.K. Micropropagation of gerbera: lipid peroxidation and antioxidant enzyme activities during acclimatization process. Acta Physiol Plant 30, 325–331 (2008). https://doi.org/10.1007/s11738-007-0125-3

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  • DOI: https://doi.org/10.1007/s11738-007-0125-3

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