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Daily rhythm of MnSOD in the C3-CAM intermediate Clusia fluminensis Planch. et Triana.

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Abstract

The C3-CAM intermediate plant Clusia fluminensis under well-watered at low light conditions opens stomata during the light period. In leaf extracts of this plant we have found two copper-zinc superoxide dismutases (CuZnSODs) and two manganese SODs: MnSOD-like protein (MnSOD II) and MnSOD I. Daily rhythm of the MnSOD I shows maximum activity during the afternoon hours and it is accompanied by only a very small tendency to increase in catalase (CAT) activity and lowering of citrate level.

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Abbreviations

CAT:

Catalase

DTT:

Dithiothreitol

EDTA:

Ethylenediaminetetraacetic acid

MnSOD:

Mn-superoxide dismutase

CuZnSOD:

CuZn-superoxide dismutase

PAGE:

Polyacrylamide gel electrophoresis

PEPC:

Phosphoenolpyruvate carboxylase

PPFD:

Photosynthetic photon flux density

PVPP:

Polyvinylpolypyrrolidone

ROS:

Reactive oxygen species

SDS:

Sodium dodecyl sulphate

TCA:

Tricarboxylic acid cycle

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Acknowledgments

This work was supported by the Deutsche Forschungsanstalt für Luft und Raumfahrt (DLR) Germany, and EU grant Qol-2001 Integr. to the Institute of Plant Physiology Polish Academy of Science. This study was partly supported by the Polish MNiSW grant N30308331/2685.

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Authors and Affiliations

  1. Institute of Biology, Pedagogical University, ul. Podbrzezie 3, 31-054, Kraków, Poland

    A. Kornas, K. Gawronska & Z. Miszalski

  2. Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239, Kraków, Poland

    I. Ślesak & Z. Miszalski

  3. Institute of Botany, Darmstadt University of Technology, Schnittspahnstrasse 3-5, 64287, Darmstadt, Germany

    E. Fischer-Schliebs

Authors
  1. A. Kornas
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  2. I. Ślesak
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  3. K. Gawronska
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  4. E. Fischer-Schliebs
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  5. Z. Miszalski
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Corresponding author

Correspondence to Z. Miszalski.

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Communicated by Z. Krupa.

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Kornas, A., Ślesak, I., Gawronska, K. et al. Daily rhythm of MnSOD in the C3-CAM intermediate Clusia fluminensis Planch. et Triana.. Acta Physiol Plant 29, 369–374 (2007). https://doi.org/10.1007/s11738-007-0046-1

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  • DOI: https://doi.org/10.1007/s11738-007-0046-1

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