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The role of the photosynthetic apparatus in cold acclimation of Lolium multiflorum. Characteristics of novel genotypes low-sensitive to PSII over-reduction

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Abstract

During cold acclimation by higher plants, temperature perception via changes in redox state of Photosystem II (PSII) and subsequent acclimation of the photosynthetic apparatus to cold is very important for achieving freezing tolerance. These properties were studied in two groups (A and B) of the same backcross 3 (BC3) progeny derived from a triploid hybrid of Festuca pratensis (2×) × Lolium multiflorum (4×) backcrossed three times onto diploid L. multiflorum cultivars. Leaves of Group A plants formed at 20°C at medium-low light were unable to acclimate their photosynthetic apparatus to cold. Compared to Group B, the Group A plants were also more frost sensitive. This acclimation ability correlated with the freezing tolerance of the plants. However, leaves of the same Group A plants developed at 20°C, but under higher-light conditions had increased ability to acclimate their photosynthetic apparatus to cold. It was concluded that Group A plants may have impaired PSII temperature perception, and this then resulted in their poor capability to cold acclimate.

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Abbreviations

ABS/CS:

Light energy absorbed by leaf cross-section

ca:

Cold acclimated plants

AREA:

Pool size of electron acceptors from PSII (proportional to the oxidized plastoquinone pool)

car:

Carotenoids (pool)

chl:

Chlorophyll

DIo/CS:

Amount of energy dissipated in PSII reaction centres per cross-section of the leaf

ETo/CS:

Quantum yield of photosynthetic electron transport chain after Q A per cross-section of the leaf

F 0 :

Fluorescence of leaves in the dark when all PSII reaction centres are open

\( {F}\ifmmode{'}\else$'$\fi_{{\text{0}}} \) :

Fluorescence in leaves previously exposed to light darkened just before measurement

F m and \( {F}\ifmmode{'}\else$'$\fi_{{\text{m}}} \) :

Fluorescence when all PSII reaction centres are closed in dark- and light-exposed leaves, respectively

F s :

Steady state fluorescence in light-exposed leaves

F t :

Freezing tolerance

F v and \( {F}\ifmmode{'}\else$'$\fi_{{\text{v}}} \) :

Variable fluorescence in dark- and light-adapted leaves, respectively (F v = F m − F 0, \( {F}\ifmmode{'}\else$'$\fi_{{\text{v}}} = {F}\ifmmode{'}\else$'$\fi_{{\text{m}}} - {F}\ifmmode{'}\else$'$\fi_{0} \))

F v:F m :

Maximum quantum yield of PSII

\( {F}\ifmmode{'}\else$'$\fi_{{\text{v}}} {\user2{:}}{F}\ifmmode{'}\else$'$\fi_{{\text{m}}} \) :

PSII antenna trapping efficiency

fw:

Fresh weight

na:

Non-acclimated plants

PPFD:

Photosynthetic photon flux density

PSII:

Photosystem II

ϕPSII:

Current quantum yield of PSII

NPQ:

Non-photochemical quenching of chlorophyll a fluorescence

QA :

The first stable electron acceptor in PSII

q P :

Photochemical quenching of chlorophyll a fluorescence

RC/CSo and RC/CSm :

Numbers of active reaction centres in the state of fully oxidized and reduced PSII reaction centre, respectively

TRo/CS:

Quantum yield of primary photochemistry (from reaction centre to Q A) per cross-section of the leaf

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Acknowledgment

This study was performed with the financial support of European Commission: SAGES project (QLK5-CT-2000-00764).

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Authors and Affiliations

  1. Faculty of Agriculture and Economics, Department of Plant Physiology, Agricultural University of Cracow, Podłużna 3, 30-239, Kraków, Poland

    M. Rapacz, D. Gąsior & J. Kościelniak

  2. Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland

    A. Kosmala & Z. Zwierzykowski

  3. Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, SY23 3EB, UK

    M. W. Humphreys

Authors
  1. M. Rapacz
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  2. D. Gąsior
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  3. J. Kościelniak
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  4. A. Kosmala
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  5. Z. Zwierzykowski
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  6. M. W. Humphreys
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Correspondence to M. Rapacz.

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Communicated by F. Dubert.

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Rapacz, M., Gąsior, D., Kościelniak, J. et al. The role of the photosynthetic apparatus in cold acclimation of Lolium multiflorum. Characteristics of novel genotypes low-sensitive to PSII over-reduction. Acta Physiol Plant 29, 309–316 (2007). https://doi.org/10.1007/s11738-007-0040-7

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  • DOI: https://doi.org/10.1007/s11738-007-0040-7

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