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Exogenous abscisic acid increases stability of polysomes in embryos of triticale caryopses during germination

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Abstract

Some posttranslational processes that occur in embryos of germinating triticale caryopses treated with different concentrations of abscisic acid (ABA) were examined. ABA increased the ratio of cytoskeleton-bound polysomes in the total population of polysomes and depressed the share of free and membrane-bound polysomes. Using exogenous RNase, stability of the total polysomal population as well as each polysomal fraction was investigated. The total extractable polysomes isolated from embryonic tissues of germinating triticale caryopses treated with ABA were more stable than the polysomes isolated from the control sample caryopses. The contribution of the polysomes that were not digested by RNase was increased by higher concentrations of ABA applied during germination. At high concentrations of ABA (50, 100 µM), the quantitative contribution of polysomes in the total ribosomal fraction was almost 100% of the amount of polysomes before digestion and the modifications observed consisted mainly of the shift of the so-called heavy polysomes towards light polysomes, containing a few ribosomes. Within each polysomal population, cytoskeleton-bound polysomes (CBP and CMBP) were the most stable, which may imply that the bonds between polysomes and these protein filaments, created in all eukaryotic cells increased their stability. It is assumed that mRNAs are stabilised or destabilised by interaction of proteins with their various sequences. A plant hormone may depress or elevate the quantities of these proteins, thus regulating the stability of different mRNAs. The results confirm the multi-faceted mechanism of ABA-induced response, where one of the constituents is the effect of ABA on the stability of mRNAs molecules. The co-ordinated regulation of mRNAs synthesis and their stability provide plants with improved adaptability.

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Abbreviations

ABA:

abscisic acid

CBP:

cytoskeleton-bound polysomes

CMBP:

cytoskeleton-membrane-bound polysomes

EGTA:

ethylene glycol-bis (β-aminoethyl ether)

N,N,N′,N′:

tetraacetic acid

FP:

free polysomes

GA:

gibberellic acid

HEPES:

N-2-hydroxyethylpiperazine-N′2′ethanesulfonic acid

KOAc:

potassium acetate

MBP:

membrane-bound polysomes

Mg(OAc)2 :

magnesium acetate

PMSF:

phenylmethylsulfonyl fluoride

PTE:

polyoxyethylene-10-tridecyl ether

RNase:

ribonuclease

Tris:

tris-(hydroxymethyl) aminomethane

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biology, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn-Kortowo, M. Oczapowskiego St. 1A, Poland

    Stanisław Weidner, Marta Każarnowicz & Ewa Frączek

  2. Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima St. 10, P.O. Box 55, 10-718, Olsztyn 5, Poland

    Ryszard Amarowicz & Magdalena Karamać

Authors
  1. Stanisław Weidner
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  2. Marta Każarnowicz
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  3. Ewa Frączek
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  4. Ryszard Amarowicz
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  5. Magdalena Karamać
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Correspondence to Stanisław Weidner.

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Stanisław, W., Marta, K., Ewa, F. et al. Exogenous abscisic acid increases stability of polysomes in embryos of triticale caryopses during germination. Acta Physiol Plant 28, 627–634 (2006). https://doi.org/10.1007/s11738-006-0059-1

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  • DOI: https://doi.org/10.1007/s11738-006-0059-1

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