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Differential response to paraquat induced oxidative stress in two rice cultivars on antioxidants and chlorophyll a fluorescence

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Abstract

The responses of antioxidative system and photosystem II photochemistry of rice (Oryza sativa L.) to paraquat induced oxidative stress were investigated in a chilling-tolerant cultivar Xiangnuo no. 1, and a chilling-susceptible cultivar, IR-50. Electrolyte leakage and malondialdehyde (MDA) content of Xiangnuo no. 1 were little affected by paraquat, but they increased in IR-50. After paraquat treatment, superoxide dismutase (SOD) activity remained high in Xiangnuo no. 1, while it declined in IR-50. Activities of catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) declined with oxidative stress in both cultivars, but Xiangnuo no. 1 had higher GR activity than IR-50. Under paraquat induced oxidative stress, ascorbic acid (AsA) and reduced glutathione (GSH) concentrations remained high in Xiangnuo no. 1, but decreased in IR-50. The results indicated that higher activities of SOD and GR and higher contents of AsA and GSH in Xiangnuo no. 1 under paraquat induced oxidative stress were associated with its tolerance to paraquat, while paraquat induced damage to IR-50 was related to decreased activities of SOD, APX and GR and contents of AsA and GSH. F v/F m, Φ PSII, and qP remained high in Xiangnuo no. 1, while they decreased greatly in IR-50 under paraquat induced oxidative stress.

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Abbreviations

APX:

Ascorbate peroxidase

AsA:

Ascorbic acid

CAT:

Catalase

DTNB:

5,5′-Dithio-bis(2-nitrobenzoic acid)

Fv/Fm:

Maximum quantum efficiency of PSII

GSH:

Glutathione, reduced form

GSSG:

Glutathione

GR:

Glutathione reductase

MDA:

Malondialdehyde

PSII:

Photosystem II

qP :

Photochemical quenching

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TBA:

Thiobarbituric acid

Φ PSII :

Quantum efficiency of PSII

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Acknowledgments

The project was funded by grants from the Natural Science Foundation of China (30370131) and the Natural Science Foundation of Guangdong Province (Grant No. 020979).

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Authors and Affiliations

  1. College of Life Science, South China Agricultural University, Guangzhou, 510642, China

    Zhenfei Guo, Shaoyun Lu, Zhao Yaqing & Qiao Zhong

  2. Pharmacy College, Jinan University, Guangzhou, 510632, China

    Meiyan Huang

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  1. Zhenfei Guo
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  2. Meiyan Huang
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  3. Shaoyun Lu
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  4. Zhao Yaqing
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  5. Qiao Zhong
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Correspondence to Zhenfei Guo.

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Communicated by G. Bartosz.

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Guo, Z., Huang, M., Lu, S. et al. Differential response to paraquat induced oxidative stress in two rice cultivars on antioxidants and chlorophyll a fluorescence. Acta Physiol Plant 29, 39–46 (2007). https://doi.org/10.1007/s11738-006-0007-0

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  • DOI: https://doi.org/10.1007/s11738-006-0007-0

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