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Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation

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Abstract

The gene fusion system was used to study UV light-control of PS PAL1 and PS PAL2 genes encoding phenylalanine ammonia-lyase of pea. The induction of pea PAL promoters was analysed in transgenic tobacco plants. Binary plasmids (derivatives of pBI101.2 vector) containing 5′ regulatory fragments of PS PAL1 and PS PAL2 linked to reporter genes (GUS,LUC) were constructed. The analyses were performed with the use of single constructs (containing one variant of PS PAL promoter and one reporter gene) and dual constructs (containing both PS PAL1 and PS PAL2 promoters connected with different reporter genes). The use of dual constructs enabled the evaluation of both PS PAL promoters activity in the same plant. The analyses of in vitro grown plants have shown that both PAL promoters are strongly induced in leaves subjected to UV radiation. In some cases, the UV-stimulated expression exceeded the exposed areas. This phenomenon was observed more often in the leaves of plants containing the PS PAL1::GUS than PS PAL2::GUS construct. Removal of boxes 2, 4, 5 from PS PAL1 promoter and deletion of its 5′ end region (-339 to -1394) decreases the level of gene expression but does not eliminate its responsiveness to UV.

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References

  • Appert CH., Logemann E., Hahlbrock K., Schmid J., Amrhein N. 1994. Structural and catalytic properties of the four phenylalanine ammonia-lyase isoenzymes from parsley (Petroselium crispum Nym.). Eur. J. Biochem. 225: 491–499.

    Article  PubMed  CAS  Google Scholar 

  • Barnes W.M. 1990. Variable patterns of expression of luciferase in transgenic tobacco leaves. Proc. Natl. Acad. Sci. USA 87: 9183–9187.

    Article  PubMed  CAS  Google Scholar 

  • Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

    Article  PubMed  CAS  Google Scholar 

  • Camm E.L., Towers G.H.N. 1973. Phenylalanine ammonia Lyase. Phytochemistry 12: 961–973.

    Article  CAS  Google Scholar 

  • Cramer C.L., Edwards K., Dron M., Liang X., Dildine S.L., Bolwell G.P., Dixon R.A., Lamb CH.J., Schuch W. 1989. Phenylalanine ammonia-lyase gene organization and structure. Plant Mol. Biol. 12: 367–383.

    Article  CAS  Google Scholar 

  • Gray-Mitsumune M., Molitor E.K., Cukovic D., Carlson J.E., Douglas C.J. 1999. Developmentally regulated patterns of expression directed by poplar PAL promoters in transgenic tobacco and poplar. Plant Mol. Biol. 39: 657–669.

    Article  PubMed  CAS  Google Scholar 

  • Hauffe K.D., Paszkowski U., Schulze-Lefert P., Hahlbrock K., Dangl J.L., Douglas C.J. 1991. A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco. Plant Cell 3: 435–443.

    Article  PubMed  CAS  Google Scholar 

  • Jangaard N.O. 1974. The characterization of phenylalanine ammonia-lyase from several plant species. Phytochemistry 13: 1765–1768.

    Article  CAS  Google Scholar 

  • Joos H-J, Hahlbrock K. 1992. Phenylalanine ammonia-lyase in potato (Solanum tuberosum L.). Eur. J. Biochem. 204: 621–629.

    Article  PubMed  CAS  Google Scholar 

  • Kao Y-Y., Harding S.A., Tsai CH-J. 2002. Differential Expression of two distinct phenylalanine ammonia-lyase genes in condensed tannin-accumulating and lignifying cells of quaking aspen. Plant Physiol. 130: 796–807.

    Article  PubMed  Google Scholar 

  • Kapusta J., Mol R., Ko czak-Islam I., Burza W. 1990. Transformacja ro lin za po rednictwem Agrobacterium tumefaciens i Agrobacterium rhizogenes. W: Transformowanie i regeneracja ro lin. Pod red. Legocki AB. Instytut Chemii Bioorganicznej PAN

  • Kato H., Wada M., Muraya K., Malik K., Shiraishi T., Ichinose Y., Yamada T. 1995. Characterization of nuclear factors for elicitor-mediated activation of the promoter of the pea phenylalanine ammonia-lyase gene 1. Plant Physiol. 108: 129–139.

    Article  PubMed  CAS  Google Scholar 

  • Kawamata S., Yamada T., Tanaka Y., Sriprasertsak P., Kato H., Ichinose Y., Kato H., Shiraishi T., Oku H. 1992. Molecular cloning of phenylalanine ammonia-lyase c DNA from Pisum sativum. Plant Mol. Biol. 20: 167–170.

    Article  PubMed  CAS  Google Scholar 

  • Kawamata S., Shimoharai K., Imura Y., Ozaki M., Ichinose Y., Shiraishi T., Kunoh H., Yamada T. 1997. Temporal and spatial pattern of expression of the pea phenylalanine ammonia-lyase genel promoter in transgenic tobacco. Plant Cell Physiol. 38: 792–803.

    PubMed  CAS  Google Scholar 

  • Koukol J., Conn E.E. 1961. The metabolism of aromatic compounds in higher plants: IV. Purification and properties of phenylalanine deaminase of Hordeum vulgare. J. Biol. Chem. 236: 2692–2698.

    PubMed  CAS  Google Scholar 

  • Leyva A., Liang X., Pintor-Toro J.A., Dixon R.A., Lamb CH.J. 1992. Cis-element combinations determine phenylalanine ammonia-lyase gene tissue-specific expression patterns. Plant Cell 4: 263–271.

    Article  PubMed  CAS  Google Scholar 

  • Loake G., Faktor O., Lamb CH.J., Dixon R.A. 1992. Combination of H-box [CCTACC(N7)CT] and G-box (CACGTG) cis elements is necessary for feed-forward stimulation of a chalcone synthase promoter by the phenylpropanoid-pathway intermediate p-coumaric acid. Proc. Natl. Acad. Sci. USA 89: 9230–9234.

    Article  PubMed  CAS  Google Scholar 

  • Lois R., Dietrich A., Hahlbrock K., Schultz W. 1989. A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements. EMBO J. 8: 1641–1648.

    PubMed  CAS  Google Scholar 

  • Michalczyk D.J., Górecki R.J. 1997. Regulation of pea PAL1 promoter in transgenic tobacco plants. Physiol. Plant. 19: 223.

    Google Scholar 

  • Murakami Y., Ichinose Y., Shiraishi T., Yamada T. 1997. Functional analysis of the putative cis-elements involved in activation by elicitor or UV and deactivation by suppressor in the promoter of a pea gene for phenylalanine ammonia-lyase (PS PAL1). Plant Cell Physiol. 38: 1403–1408.

    CAS  Google Scholar 

  • Murashige T., Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.

    Article  CAS  Google Scholar 

  • Nadolska-Orczyk A. 2000. Regeneracja in vitro i transformacja genetyczne ro lin motylkowych grubonasiennych. IHAR, Radzików 6: 30–31.

    Google Scholar 

  • Nishiuchi T., Hamada T., Kodama H., Iba K. 1997. Wounding changes the spatial expression pattern of the arabidopsis plastid omega-3 fatty acid desaturase gene (FAD7) through different signal transduction pathways. Plant Cell 9: 1701–1712.

    Article  PubMed  CAS  Google Scholar 

  • Pellegrini L., Rohfritsch O., Fritig B., Legrand M. 1994. Phenylalanine ammonia-lyase in tobacco. Plant Physiol. 106: 877–886.

    Article  PubMed  CAS  Google Scholar 

  • Pniewski T. 2001. Ekspresja w ro linach antygenów wirusowych o potencjalnym znaczeniu aplikacyjnym. Praca wykonana w Pracowni Biologii Molekularnej Ro lin Instytutu Chemii Bioorganicznej PAN w Poznaniu, pod kierunkiem prof. dr hab. Andrzeja B. Legockiego.

  • Roger D., Lacoux J., Lamblin F., Gaillet D., Dauchel H., Klein D., Balangé A.P., David A., Lainé E. 2001. Isolation of flax pectin methylesterase promoter and its expression in transgenic tobacco. Plant Science 160: 713–721.

    Article  PubMed  CAS  Google Scholar 

  • Rushton P.J., Torres J.T., Parniske M, Wernert P, Hahlbrock K, Somssich E. 1996. Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes. EMBO J. 15: 5690–5700.

    PubMed  CAS  Google Scholar 

  • Seki H., Nagasugi Y., Ichinose Y., Shiraishi T., Yamada T. 1999. Changes in in vivo DNA-protein interactions in pea phenylalanine ammonia-lyase and chalcone synthase gene promoter induced by fungal signal molecules. Plant Cell Physiol. 40: 88–95.

    PubMed  CAS  Google Scholar 

  • Sriprasertsak P., Salamah A., Imura Y., Ichinose Y., Shiraishi T., Yamada T. 1999. Expression of the chimeric pea PS PAL2 promoter in transgenic tobacco in response to fungal ingress and injury. Ann. Phytopathol. Soc. Jpn. 65: 123–130.

    CAS  Google Scholar 

  • Wada M., Kato H., Malik K., Sriprasertsak P., Ichinose Y., Shiraishi T., Yamada T. 1995. A supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encoding phenylalanine ammonia-lyase. J. Mol. Biol. 249: 513–519.

    Article  PubMed  CAS  Google Scholar 

  • Yamada T., Tanaka Y., Sriprasertsak P., Kato H., Hashimoto T., Kawamata S., Ichinose Y., Kato H., Shiraishi T., Oku H. 1992. Phenylalanine ammonia-lyase genes from Pisum sativum: structure, organ-specific expression and regulation by fungal elicitor and suppressor. Plant Cell Physiol. 33: 715–725.

    CAS  Google Scholar 

  • Yamada T., Sriprasertsak P., Kato H., Hashimoto T., Shimizu H., Shiraishi T. 1994. Functional analysis of the promoters of phenylalanine ammonia-lyase genes in pea. Plant Cell Physiol. 35: 917–926.

    PubMed  CAS  Google Scholar 

  • Yan X., Gonzales R.A., Wagner G.J. 1997. Gene fusions of signal sequences with a modified beta-glucuronidase gene results in retention of the beta-glucuronidase protein in the secretory pathway/plasma membrane. Plant Physiol. 115: 915–924.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 1A, 10-719, Olsztyn

    Wioletta Ewa Pluskota, Dariusz Jan Michalczyk & Ryszard Józef Górecki

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  1. Wioletta Ewa Pluskota
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  2. Dariusz Jan Michalczyk
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  3. Ryszard Józef Górecki
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Correspondence to Wioletta Ewa Pluskota.

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Pluskota, W.E., Michalczyk, D.J. & Górecki, R.J. Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation. Acta Physiol Plant 27, 229–236 (2005). https://doi.org/10.1007/s11738-005-0027-1

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  • DOI: https://doi.org/10.1007/s11738-005-0027-1

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