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Effect of hypoxia on photosynthetic activity and antioxidative response in gametophores of Mnium undulatum

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Abstract

The effects of hypoxia caused by complete submerging of Mnium undulatum gametophores in water, on their photosynthetic activity and the activity of two antioxidative enzymes: superoxide dismutase (SOD) and catalase (CAT) were investigated. The net photosynthesis was strongly inhibited throughout the experiment, and the strong drop in the maximum quantum yield of the PSII (Fv/Fm) was also observed. Three classes of SOD: MnSOD, FeSOD, Cu/ZnSOD and three isoforms of Cu/ZnSOD were identified. A significant decrease in activity of MnSOD, FeSOD and one Cu/ZnSOD isoform was observed after 24 and 48 h of hypoxia. FeSOD activity decreased already after 1 h of submerging in water and its activity remained at the low level during whole period of the experiment. CAT activity was also strongly inhibited in response to hypoxia stress. The obtained results suggest relationships between photosynthetic activity and antioxidative system in M. undulatum gametophores under oxygen deficiency stress.

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Abbreviations

BSA:

bovine serum albumin

CAT:

catalase

DTT:

dithiothreitol

EDTA:

ethylenediamine tetraacetic acid

EGTA:

ethyleneglycol-bis(beta-aminoethylether)-N,N′-tetracet ic acid

Fm :

maximum chlorophyll a fluorescence yield

Fo :

minimum chlorophyll a fluorescence yield

Fv :

the difference between F m and F o

Fv/Fm :

maximum quantum yield of PSII

NBT:

nitroblue tetrazolium salt

PAGE:

polyacrylamide gel electrophoresis

PAR:

photosynthetically active radiation

Pn :

net photosynthesis intensity

PSII:

photosystem II

ROS:

reactive oxygen species

SOD:

superoxide dismutase

Tricine:

N-[tris-(hydroxymethyl)methyl]glycine

Tris:

tris(hydroxymethyl)aminomethane

TEMED:

N,N,N′,N′-tetramethylethylenediamine

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Authors and Affiliations

  1. Department of Plant Physiology, Institute of Biology, Pedagogical Academy, Podbrzezie 3, 30-054, Kraków, Poland

    Andrzej Rzepka & Jan Krupa

  2. Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239, Kraków, Poland

    Ireneusz lesak

Authors
  1. Andrzej Rzepka
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  2. Jan Krupa
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  3. Ireneusz lesak
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Rzepka, A., Krupa, J. & lesak, I. Effect of hypoxia on photosynthetic activity and antioxidative response in gametophores of Mnium undulatum . Acta Physiol Plant 27, 205–212 (2005). https://doi.org/10.1007/s11738-005-0024-4

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  • DOI: https://doi.org/10.1007/s11738-005-0024-4

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