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Expressed sequence tags and their application for plant research

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Abstract

Expressed Sequence Tags (ESTs) are short, usually unedited sequences obtained by single-pass sequencing of cDNA clones from any cDNA library. Analyzing and comparing ESTs can provide information on gene expression, function and evolution. Large-scale EST sequencing has become an attractive alternative to plant genome sequencing. Currently, plant EST collections comprise over 3.8 million sequences from about 200 species. They have proved to be a valuable tool for gene discovery and plant metabolism analysis. Several plant-specific EST databases have been created which provide access to sequence data and bioinformatics-based tools for data mining. Searching EST collections allows pre-selection of genes for preparing cDNA arrays, targeted to bring maximum information on specialized processes, like stress response, symbiotic nitrogen fixation etc. Also, ESt-based molecular markers such as SNP, SSR, and indels are fast developing tools for breeders and researchers.

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Abbreviations

EST:

Expressed Sequence Tag

SNF:

symbiotic nitrogen fixation

SNP:

single nucleotide polymorphism

SSR:

simple sequence repeat

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Pozna, Poland

    Agnieszka Kisiel & Jan Podkowi ski

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  1. Agnieszka Kisiel
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  2. Jan Podkowi ski
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Correspondence to Agnieszka Kisiel.

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Kisiel, A., Podkowi ski, J. Expressed sequence tags and their application for plant research. Acta Physiol Plant 27, 157–161 (2005). https://doi.org/10.1007/s11738-005-0019-1

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  • DOI: https://doi.org/10.1007/s11738-005-0019-1

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