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Two-dimensional leaf isozyme patterns of Aegilops kotschyi ’ Secale cereale amphiploids

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Abstract

Esterase, peroxidase, shikimic dehydrogenase, malic dehydrogenase and diaphorase isozymes in leaves of the amphiploids Aegilops kotschyi × Secale cereale and their parental forms (Ae. kotschyi and S. cereale) were analyzed using two-dimensional gel electrophoresis in non-denaturing conditions. In the amphiploid isozymess were detected, which were not detected in leaves of parental plants. In contrast, some parental isozymes were not detected in refer to the gels of the amphiploids. Detection of new isoforms in the amphiploids and no detection of some parental isoforms is discussed considering the recombination, gene suppression, acting of inhibitors, chromosome translocation and also refer to the previous results of the electrophoretic analysis of proteins and enzymes of Aegilops sp. × S. cereale amphiploids.

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Abbreviations

EST:

esterase

PX:

peroxidase

SKDH:

shikimic dehydrogenase

MDH:

malic dehydrogenase

DIA:

diaphorase

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Authors and Affiliations

  1. Institute of Plant Genetics, Polish Academy of Sciences, Strzeszy ska 34, 60-479, Pozna, Poland

    Andrzej Kalinowski & Barbara Wojciechowska

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  1. Andrzej Kalinowski
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  2. Barbara Wojciechowska
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Kalinowski, A., Wojciechowska, B. Two-dimensional leaf isozyme patterns of Aegilops kotschyi ’ Secale cereale amphiploids. Acta Physiol Plant 26, 75–84 (2004). https://doi.org/10.1007/s11738-004-0047-2

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  • DOI: https://doi.org/10.1007/s11738-004-0047-2

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