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Water-deficit-induced changes in cytoskeleton-bound and other polysomal populations in embryonic tissue during triticale caryopsis germination

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Abstract

We studied the influence of water-deficit stress on the process of formation of different polysomal populations, their abundance and stability in embryonic tissue during triticale caryopsis germination. Osmotic stress retarded the ability of seeds to germinate and decreased the content of the total ribosomal fraction in embryos. In control samples, the fraction of free polysomes was the most abundant and this population of polysomes decreased sharply in osmotic stress conditions. Water-deficit stress applied during germination profoundly changed the proportions between different polysome populations in the total ribosomal fraction of embryonic tissue. The predominant population in these conditions was the cytoskeleton-bound fraction. This may indicate an important role for cytoskeleton-bound polysomes in the synthesis of stress-induced proteins. We hypothesize that there must be an active mechanism of translational control that permits specific proteins to be synthesized despite a reduction in total protein synthesis.

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Abbreviations

ABA:

abscisic acid

CBP:

cytoskeleton-bound polysomes

CMBP:

cytoskeleton-membrane-bound polysomes

EGTA:

ethylene glycol-bis (β-aminoethyl ether)

N,N,N′,N′:

tetraacetic acid

FP:

free polysomes

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

KOAc:

potassium acetate

MBP:

membrane-bound polysomes

Mg(OAc)2 :

magnesium acetate

PMSF:

phenylmethylsulfonyl fluoride

PEG:

polyethylene glicol

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego St. 1A, 10-1957, Olsztyn-Kortowo, Poland

    Monika Kosowska, Ewa Fr czek & Stanisław Weidner

  2. Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima St. 10, P.O. Box 55, 10-718, Olsztyn 5, Poland

    Ryszard Amarowicz & Magdalena Karama

  3. Lab. of Molec. Cell Biol., College of Agriculture, Ehime University, 3-5-7 Tarumii, Matsuyama, 7901, Ehime, Japan

    Shunnosuke Abe

Authors
  1. Monika Kosowska
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  2. Ewa Fr czek
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  3. Ryszard Amarowicz
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  4. Magdalena Karama
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  5. Shunnosuke Abe
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  6. Stanisław Weidner
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Correspondence to Stanisław Weidner.

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Kosowska, M., Fr czek, E., Amarowicz, R. et al. Water-deficit-induced changes in cytoskeleton-bound and other polysomal populations in embryonic tissue during triticale caryopsis germination. Acta Physiol Plant 26, 67–74 (2004). https://doi.org/10.1007/s11738-004-0046-3

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  • DOI: https://doi.org/10.1007/s11738-004-0046-3

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