Skip to main content
SpringerLink
Log in

Changes in the pattern of antioxidant enzymes in wheat exposed to water deficit and rewatering

  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Antioxidant defenses in two wheat cultivars differing in sensitivity to dehydration (YouJian (YJ-24) more sensitive than LongChun (LC-20) were analyzed during water deficit and rewatering. Resistant cultivar (LC-20) showed a higher relative water content than the sensitive cultivar (YJ-24) during the whole period of water withholding. In order to analyze the changes of antioxidant enzymes, native PAGE analysis of protein extract were performed. Wheat leaves had two isoforms of Mn-superoxide dismutase (SOD), two isoforms of Cu/Zn-SOD and one of Fe-SOD. Three catalase (CAT) isoforms were identified in the leaves of wheat. The activities of SOD and CAT isoforms were increased in two cultivars under water deficit. The intensities of SOD and CAT isoforms were slightly lower in LC-20 and increased continuously in YJ-24 after rewatering. Peroxidase (POD) isoforms were significantly increased during the whole dehydration-rehydration period. Three ascorbate peroxidase (APX) isoforms were present in gel. APX-1 and APX-3 were enhanced during water deficit and decreased during rewatering in LC-20. In YJ-24 only the activities of APX-2 were increased under water deficit. Seven isoforms of glutathione reductase (GR) were detected in the native gel. Activities of most of GR isoforms were higher in tolerant (LC-20) than in sensitive cultivar (YJ-24). Different isoforms of GR in two wheat cultivars behaved differently under water deficit and rewatering. These results collectively suggest that water deficit activates the SOD, CAT and ascorbate-glutathione cycle in wheat leaves. The response of enzyme isoforms to drought is not the same for all isoforms of antioxidant enzymes in two wheat cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alscher R.G., Hess J.L. 1993. Antioxidants in higher plants. In Foyer, C.H. and Mullineaux, P.M. (eds.). Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants. Pp 59–87. CRC Press, Boca Raton, FL.

    Google Scholar 

  • Arora A., Satram R.K., Srirastava G.L. 2002. Oxidative stress and antioxidative system in plants. Curr. Sci., 82: 1227–1238.

    CAS  Google Scholar 

  • Asada K. 1992. Ascorbate peroxidases — a hydrogen peroxide scavenging enzyme in plants. Physiol. Plant., 55: 235–241.

    Article  Google Scholar 

  • Asada K. 1994. Cause of Photooxidative stress and Amelioration of Defence Systems in Plants. In Foyer, C.H. and Mullineaux, P.M. (eds), Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants. Pp 77–104. CRC Press, Boca Raton, FL.

    Google Scholar 

  • Badiani M., Biasi De M.G., Colognola M., Artemi F. 1990. Catalase, peroxidase and superoxide dismutase activies in seedlings submitted to increasing water deficit. Agrochimica 34: 90–102.

    CAS  Google Scholar 

  • Beauchamp C., Friclorich I. 1971. Superoxide dismutase: improved assays and assay applicable to acrylamide gels. Anal. Biochem., 44: 276–287.

    Article  PubMed  CAS  Google Scholar 

  • Beyer W.F., Fridovich I. 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal. Biochem., 161: 559–566.

    Article  PubMed  CAS  Google Scholar 

  • Bowler C., Van C.W., Van M.M., Inze D. 1994. Superoxide dismutase in plants. CRC Crit. Rev. Plant Sci., 13: 199–218.

    CAS  Google Scholar 

  • Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantity of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–254.

    Article  PubMed  CAS  Google Scholar 

  • Burke J.J., Gamble P.E., Hatfieid J.L., Quisenberry J.E. 1985. Plant morphological and biochemical responses to field water deficits. I. Responses of glutathione reductase activity and paraquat sensitivity. Plant Physiol., 79: 415–419.

    Article  PubMed  CAS  Google Scholar 

  • Creissen G.P., Edwards E.A., Mullineaux P.M. 1994. Glutathione reductase and ascorbate peroxidase. In: Foyer C.H., Mullineaux, P.M. (eds) Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants. Pp 343–364. CRC Press, Boca Raton, FL.

    Google Scholar 

  • Donahue J.L., Okpodu C.M., Cramer C.L., Grabau E.A., Alscher R.G. 1997. Responses of antioxidants to paraquat in pea leaves: relationships to resistance. Plant Physiol., 113: 249–257.

    PubMed  CAS  Google Scholar 

  • Edwards E.A., Enard C., Creissen G.P., Mullineaux P.M. 1994. Synthesis and properties of glutathione reductase in stressed peas. Planta 192: 137–143.

    CAS  Google Scholar 

  • Foyer C.H., Descouvieres P., Kunert K.J. 1994a. Protection against oxygen radicals: an important defense mechanism studied in transgenic plants. Plant Cell Environ., 17: 507–523.

    Article  CAS  Google Scholar 

  • Foyer C.H., Lelandais M., Kenert K.J. 1994b. Photooxidative stress in plants. Physiol. Plant., 92: 697–717.

    Article  Google Scholar 

  • Gaspar T., Penel C., Thorpe T., Greppin H., (eds) 1982. Peroxidases 1970–1980. A survey of their biochimical roles in higher plants. University of Geneva Press, Geneva, Switzerland. Pp 89–112.

    Google Scholar 

  • Gogorcena Y., Iturbe-Ormaetxe I., Escuredo P.R., Becana M. 1995. Antioxidant defense against activated oxygen in pea nodules subjected to water stress. Plant Physiol., 108: 753–759.

    PubMed  CAS  Google Scholar 

  • Laemmli U.K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680–685.

    Article  PubMed  CAS  Google Scholar 

  • Luna M., Badiani M., Felice M., Artemi F., Germanni G. 1985. Selective enzyme inactivation under water stress in maize (Zea mays L.) and wheat (Triticum aestium L.) seedlings. Environ. Exp. Bot., 25: 153–156.

    Article  CAS  Google Scholar 

  • Malan C., Greyling M. M., Gressel J. 1990. Correlation between CuZn-superoxide dismutase and glutathione reductase, and environmental and xenobiotic stress tolerance in maize inbreeds. Plant Sci., 69: 157–166.

    Article  CAS  Google Scholar 

  • Mittler R., Zilinskas B.A. 1993. Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate dependent reduction of nitroblue tetrazolium. Anal. Biochem., 212: 540–546.

    Article  PubMed  CAS  Google Scholar 

  • Mittova V., Volokita M., Guy M., Tal M. 2000. Activities of SOD and the ascorbate-glutathione cycle in subcellular compartment in leaves and roots of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Physiol. Plant., 110: 42–51.

    Article  CAS  Google Scholar 

  • Navari-Izzo F., Quartacci M.F., Sgherri C.L.M. 1999. Superoxide and hydroxyl radical generation, and superoxide dismutase in PSII membrane fragments from wheat. Free Rad. Res., 31: S3–9.

    Article  CAS  Google Scholar 

  • O’Kane D., Gill V., Boyd P., Burdon R. 1996. Chilling, oxidative stress and antioxidant responses in Arabidopsis thaliana callus. Planta 198: 371–377.

    Article  PubMed  CAS  Google Scholar 

  • Otter T., Polle A. 1994. The influence of apoplastic ascorbate on the activities of cell-wall associated peroxidases and NADP-oxidases in needles of Norway spruce (Picea abies L.). Plant Cell Physiol., 35: 1231–1238.

    CAS  Google Scholar 

  • Perl-Treves R., Galun E. 1991. The tomato Cu, Zn superoxide dismutase genes are developmentally regulated and respond to light and stress. Plant Mol. Biol., 17: 745–760.

    Article  PubMed  CAS  Google Scholar 

  • Polle A., Otter T., Seifert F. 1994. Apoplastic peroxidases and lignification in needles of Norway spruce (Picea abies L.). Plant Physiol., 106: 53–60.

    PubMed  CAS  Google Scholar 

  • Porcel R., Barea J.M., Ruiz-Lozano, J.M. 2003. Antioxidant activities in mycorrhizal soybean plants under drought stress and their possible relationship to the process of nodule senescence. New Phytol., 157: 135–143.

    Article  CAS  Google Scholar 

  • Rao M.V., Paliyath G., Ormrod D.P. 1996. Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol., 110: 125–136.

    Article  PubMed  CAS  Google Scholar 

  • Quartacci M.F., Navari-Izzo F. 1992. Water stress and free radical mediated changes in sunflower seedlings. J. Plant Physiol., 142: 621–625.

    Google Scholar 

  • Quartacci M.F., Sgheeri C.L.M., Navari-Izzo F. 1995. Lipid composition and protein dynamics in thylakoids of two wheat cultivars differently sensitive to drought. Plant Physiol., 108: 191–197.

    PubMed  CAS  Google Scholar 

  • Quartacci M.F., Sgheeri C.L.M., Pinzino C., Navari-Izzo F. 1994. Superoxide radical production in wheat plants differently sensitive to drought. Proc. R. Soc. Edinb., 102B: 287–290.

    Google Scholar 

  • Sairam R.K., Deshmukh P.S., Saxena D.C. 1998. Role of antioxidant systems in wheat genotypes tolerance to water stress. Biol. Plant., 41: 387–394.

    Article  CAS  Google Scholar 

  • Sairam R.K., Saxena D.C. 2000. Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. J. Agron. Crop. Sci., 184: 55–61.

    Article  CAS  Google Scholar 

  • Salin M.L., Lyon D.S. 1983. Iron-containing superoxide dismutases in eukaryotes: Localization in chloroplasts from water lily, Nuphar Iuteum. In Oxy Radical and Their Scavenger Systems. Cohen. G., Greenwald. R.A. (eds.). Pp 344–347.

  • Sergiev I., Alexieva V., Karanov E. 1997. Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants, Compt. Rend. Acad. Bulg. Sci., 51: 121–124.

    Google Scholar 

  • Sgherri C.L.M., Loggini B., Puliga S., Navari-Izzo F. 1994. Antioxidant system in Sorobolus stapfianus: changes in response to desiccation and rehydration. Phytochem., 35: 561–565.

    Article  CAS  Google Scholar 

  • Sgheeri C.L.M., Maffei M., Navari-Izzo F. 2000. Antioxidative enzymes in wheat subjected to increasing water deficit and rewatering. J. Plant Physiol., 157: 273–279.

    Google Scholar 

  • Sgherri C.L.M., Pinzion C., Navari-Izzo F. 1996. Sunflower seedlings subjected to stress by water defict: changes in O2 — production related the composition of thylakoid membranes. Physiol. Plant., 96: 446–452.

    Article  CAS  Google Scholar 

  • Slooten L., Capiau K., Van Camp W., Van Montagu M., Sybesma C., Inzé D. 1995. Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplasts. Plant Physiol., 107: 737–750.

    PubMed  CAS  Google Scholar 

  • Van Camp W., Capiau K., Van Montagu M., Inzé D., Slooten L. 1996. Ehancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fesuperoxide dismutase in the chloroplasts. Plant Physiol., 112: 1703–1714.

    Article  PubMed  Google Scholar 

  • Yu Q., Rengel Z. 1999. Drought and salinity differentially influence activities of superoxide dismutases in narrow-leafed lupins. Plant Sci., 142: 1–11.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Lab of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, P.R.China

    Zhang Feng, Guo Jin-Kui, Yang Ying-Li, He Wen-Liang & Zhang Li-Xin

  2. School of Life Science, Lanzhou University, 730000, Lanzhou, P.R.China

    Guo Jin-Kui & Zhang Li-Xin

  3. Agronomy Faculty of Gansu Agricultural University, 730070, Lanzhou, P.R.China

    Zhang Feng

Authors
  1. Zhang Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guo Jin-Kui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yang Ying-Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. He Wen-Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhang Li-Xin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhang Li-Xin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, F., Guo, JK., Yang, YL. et al. Changes in the pattern of antioxidant enzymes in wheat exposed to water deficit and rewatering. Acta Physiol Plant 26, 345–352 (2004). https://doi.org/10.1007/s11738-004-0024-9

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-004-0024-9

Key words

Search

Navigation