Abstract
Slowly activating vacuolar channels (SV), were examined in embryogenic and non-embryogenic cultures of winter wheat using a patch-clamp technique. Four different types of cultures were examined: embryogenic and non-embryogenic calli from embryos, embryogenic and non-embryogenic calli from inflorescences. In a cell-attached mode single SV channel events were recorded. Unitary conductance of single SV channels was between 37 pS and 48 pS and did not significantly depend on the kind of the culture, although it was a tendency that SV channels of embryogenic calli possessed lower unitary conductance than those of non-embryogenic. 2,4-D caused significant lowering of unitary conductance from 48±6 pS in the control culture of embryogenic embryos to 28±6 pS in vacuoles treated. The SV channel density was estimated as 0.34 µm−2.
Similar content being viewed by others
Abbreviations
- CICR:
-
calcium-induced calcium release
- SV channel:
-
slowly activating vacuolar channel
References
Allen G.J., Sanders D. 1996. Control of ionic currents in guard cell vacuoles by cytosolic and luminal calcium. Plant J. 10: 1055–1069.
Allen G.J., Sanders D. 1997. Vacuolar ion channels of higher plants. Adv. Bot. Res. 3914: 217–252.
Bertl A., Blumwald E., Coronado R., Eisenberg R., Findlay G.P., Gradmann D., Hille B., Köhler K., Kolb H.A., MacRobbie E.A.C., Meissner G., Miller C. 1992. Electrical measurements on endomembranes. Science 258: 873–874.
Brownlee C., Goddard H., Hetherington A.M., Peake L-A. 1999. Specificity and interaction of responses: Ca2+ as a signal in polarity and osmotic regulation. J. Exp. Bot. 50: 1001–1011.
Brüggemann L.I., Pottosin I.I., Schönknecht G. 1999. Cytoplasmic magnesium regulates the fast activating vacuolar cation channel. J. Exp. Bot. 50: 1547–1552.
Cvirková M., Binarová P., Cenklová V., Eder J., Macháčková I. 1999. Reinitiation of cell division and polyamine and aromatic monoamine levels in alfalfa explants during the induction of somatic embryogenesis. Physiol. Plant. 105: 330–337.
De Jong A.J., Schmidt E.D.L., de Vries S.C. 1993. Early events in higher-plant embryogenesis. Plant Mol. Biol. 22: 367–377.
Fujimura T., Komamine A. 1979. Synchronization of somatic embryogenesis in carrot suspension culture. Plant Physiol. 64: 162–164.
Hamill O.P., Marty A., Neher E., Sakmann B., Sigworth F.J. 1981. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391: 85–100.
Hedrich R., Neher E. 1987. Cytoplasmic calcium regulates voltage-dependent ion channels in plant vacuoles. Nature 329: 833–835.
Jiménez V., Bangerth F. 2001. Endogenous hormone levels in explants and embryogenic and non-embryogenic cultures of carrot. Physiol. Plant. 111: 389–395.
Król E., Trębacz K. 2000. Ways of ion channel gating in plant cells. Ann. Bot. 86: 449–469.
McAinsh M.R., Brownlee C., Hetherington A.M. 1997. Calcium ions as second messengers in guard cell signal transduction. Physiol. Plant. 100: 16–29.
Marcińska I., Dubert F., Biesaga-Kościelniak J. 1995. Transfer of ability to flower in winter wheat via callus tissue from immature inflorescences. Plant Cell Tis. Org. Cult. 41: 285–288.
Mayer U., Jurgens G. 1998. Pattern formation in plant embryogenesis: a reassessment. Sem. Cell Dev. Biol. 9: 109–110.
Michalczuk L., Ribnicky D.M., Cooke T.J., Cohen J.D. 1992 Regulation of indole-3-acetic acid biosynthetic pathway in carrot cell cultures. Plant Physiol. 100: 1346–1353.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
Pei Z-M., Ward J.M., Schroeder J.I. 1999. Magnesium sensitizes slow vacuolar channels to physiological cytosolic calcium and inhibits fast vacuolar channels in Faba bean guard cell vacuoles. Plant Physiol. 121: 977–986.
Pottosin I.I., Tikhonova L.I., Hedrich R., Schönknecht G. 1997. Slowly activating vacuolar channels can not mediate Ca2+-induced Ca2+ release. Plant J. 12: 1387–1398.
Steward F.C., Mapes M.O., Mears K. 1958. Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Am. J. Bot. 45: 705–708.
Trębacz K., Schönknecht G. 2000. Simple method to isolate vacuoles and protoplasts for patch-clamp experiments. Protopasma 213: 39–45.
Trebacz K., Simonis W., Schönknecht G. 1994. Cytoplasmic Ca2+, K+, Cl−, and NO3 − activities in the liver-wort Conocephalum conicum L. at rest and during action potentials. Plant Physiol. 106: 1073–1084.
Wake H., Umetsu H., Matsunaga T. 1995. Somatic embryogenesis and artificial seed in carrot. In: Bajaj Y.P.S. (ed). Somatic Embryogenesis and Synthetic Seed. II. Biotechnology in Agriculture and Forestry. Vol. 31. Springer-Verlag, Berlin, pp. 170–182.
Williamson R.E., Ashley C.C. 1982. Free Ca2+ and cytoplasmic streaming in the alga Chara. Nature 296: 647–651.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dziubińska, H., Filek, M., Szechyńska-Hebda, M. et al. Slow vacuolar channels of non-embryogenic and embryogenic cultures of winter wheat. Acta Physiol Plant 25, 179–184 (2003). https://doi.org/10.1007/s11738-003-0051-y
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11738-003-0051-y