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The influence of abscisic acid on different polysomal populations in embryonal tissue during pea seeds germination

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Abstract

The influence of abscisic acid (ABA) on the processes of formation of different polysomal populations, their structures and stability in embryonal tissue during pea seeds germination was studied. The contents of total ribosomal fraction increased in all samples up to 72 h of germination and then decreased. The contents of polysomal population (FP, MBP, CBP and CMBP) extracted from the embryonal tissue after 72 hrs of germination of pea seeds were then quantified. It turned out that in examined tissue of control sample, fraction of free polysomes (FP) was the most abounded. This population of polysomes in sprouts decreased after ABA treatment. FP content decreased even more when the higher ABA concentration was applied during germination. Similar changes were observed in the fraction of membrane-bound polysomes (MBP). Quite different tendencies were found, however, in forming population of the cytoskeleton-membrane-bound polysomes (CMBP). The CMBP population content in embryonal tissue increased in a dosage dependent manner with increasing concentration of ABA applied during seed germination. This indicates the important role of CMBP fraction in synthesis of specific proteins in embryos in the time when processes of seeds germination are retarded by ABA. In the final part we examined the stability of polysomes isolated from sprouts of germinating seeds in water and sprouts isolated from seeds treated with ABA (100 µM) during germination. Total polysomes isolated from embryonal tissue of germinating seeds treated with ABA showed much higher resistance to exogenous ribonuclease digestion than total polysomes of control sample. The obtained results suggest that ABA influence on different polysomal population formation also controls their stability.

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Abbreviations

ABA:

abscisic acid

CBP:

cytoskeleton-bound polysomes

CMBP:

cytoskeleton-membrane-bound polysomes

EGTA:

ethylene glycol-bis (ß-aminoethyl ether) N,N,N′,N′-tetraacetic acid

FP:

free polysomes

GA:

gibberellic acid

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

KOAc:

potassium acetate

MBP:

membrane-bound polysomes

Mg(OAc)2 :

magnesium acetate

PMSF:

phenylmethylsulfonyl fluoride

PTE:

polyoxyethylene-10-tridecyl ether

RNase:

ribonuclease

Tris:

tris-(hydroxymethyl) aminomethane

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego St. 1A, 10-1957, Olsztyn-Kortowo, Poland

    Stanisław Weidner, Ewa Frączek & Magdalena Romanowska

  2. Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima St. 10, P.O. Box 55, 10-718, Olsztyn 5, Poland

    Ryszard Amarowicz

  3. Lab. of Molec. Cell Biol., College of Agriculture, Ehime University, 3-5-7 Tarumii, Matsuyama, 7901, Ehime, Japan

    Shunnosuke Abe

  4. Botany Department, North Carolina State University, 27695-7612, Raleigh, NC, USA

    Eric Davies

Authors
  1. Stanisław Weidner
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  2. Ewa Frączek
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  3. Magdalena Romanowska
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  4. Ryszard Amarowicz
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  5. Shunnosuke Abe
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  6. Eric Davies
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Correspondence to Stanisław Weidner.

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Weidner, S., Frączek, E., Romanowska, M. et al. The influence of abscisic acid on different polysomal populations in embryonal tissue during pea seeds germination. Acta Physiol Plant 25, 5–12 (2003). https://doi.org/10.1007/s11738-003-0030-3

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  • DOI: https://doi.org/10.1007/s11738-003-0030-3

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