Abstract
Interspecific somatic hybrids between a dihaploid potato clone H-8105 susceptible to Phytophthora infestans (Mont.) de Bary and a resistant diploid tuberizing species Solanum bulbocastanum were generated and analysed. Only ten regenerants displaying the intermediate morphology with dominating characteristics of the wild parent (simple leaves, anthocyanin pigmentation) were produced in 15 weeks after a single PEG-mediated fusion event. The RAPD patterns confirmed the hybridity of all of them. The hybrids rooted poorly and grew slowly in vitro. The cytological analysis revealed a high degree of aneuploidy in the hybrids with morphological and growth anomalies in vitro, while the morphologically normal hybrids were tetraploids. All the S. bulbocastanum (+) H-8105 hybrids were unstable in culture and three of them were consequently lost during three years of propagation in vitro. The possible reasons for instability of somatic hybrids between the distantly related species are discussed.
Similar content being viewed by others
References
Austin S., Łojkowska E., Ehlenfeldt M. K., Kelman A., Helgeson J. P. 1988. Fertile interspecific somatic hybrids of Solanum: a novel source of resistance to Erwinia soft rot. Phytopathology, 78: 1216–1220.
Austin S., Pohlman J.D., Brown C.R., Mojtahedi H., Santo G.S., Douches D.S., Helgeson J.P. 1993. Interspecific somatic hybridization between Solanum tuberosum L. and S. bulbocastanum Dun. as a means of transferring nematode resistance. Am. Potato J., 70: 485–495.
Babiychuk E., Kushnir S., Gleba Y.Y. 1992. Spontaneous extensive chromosome elimination in somatic hybrids between somatically congruent species Nicotiana tabacum L. and Atropa belladonna L. Theor. Appl. Genet., 84: 87–91.
Baird E., Cooper-Bland S., Waugh R., De Maine M., Powell W. 1992. Molecular characteristics of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA (RAPD) markers. Mol. Gen. Genet., 233: 469–475.
Binding H., Jain S.M., Finger J., Mordhorst G., Nehls R., Gressel J. 1982. Somatic hybridization of an atrazine resistant biotype of Solanum nigrum and Solanum tuberosum. Part 1: Clonal variation in morphology and in atrazine sensitivity. Theor. Appl. Genet., 63: 273–277.
Cardi T., Ambrosio F.D., Consoli D., Puite K.J., Ramulu K.S. 1993. Production of somatic hybrids between frost-tolerant Solanum commersonii and S. tuberosum: characterization of hybrid plants. Theor. Appl. Genet., 87: 193–200.
Chen Y-K.H., Bamberg J.B., Palta J.P. 1999. Expression of the freezing tolerance in the interspecific F1 and somatic hybrids of potatoes. Theor. Appl. Genet., 98: 995–1004.
Deimling S., Zitzlsperger J., Wenzel G. 1988. Somatic fusion for breeding of tetraploid potatoes. Plant Breeding, 101: 181–189.
Fock I., Collonnier C., Purwito A., Luisetti J., Souvannavong V., Vedel F., Servaes A., Ambroise A., Kodja H., Ducreux G., Sihachakr D. 2000. Resistance to bacterial wilt in somatic hybrids between Solanum tuberosum and Solanum phureja. Plant Sci., 160: 165–176.
Hein T., Przewoźny T., Schieder O. 1983. Culture and selection of somatic hybrids using an auxotrophic cell line. Theor. Appl. Genet., 64: 119–122.
Helgeson J.P., Haberlach G.T. 1999. Somatic hybrids of Solanum tuberosum and related species. In: Altman A., Ziv M., Izhar S. (eds.) Plant biotechnology and in vitro biology in the 21st century. Kluwer Academic Publishers, Dordrecht, pp. 151–154.
Helgeson J.P., Haberlach G.T., Ehlenfeldt M.K., Hunt G., Pohlman J.D., Austin S. 1993. Sexual progeny of somatic hybrids between potato and Solanum brevidens: potential for use in breeding programs. Am. Potato J., 70: 437–452.
Helgeson J.P., Pohlman J.D., Austin S., Haberlach G.T., Wielgus S.M., Ronis D., Zambolim L., Tooley P., McGrath J.M., James R.M., Stevenson W.K. 1998. Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theor. Appl. Genet., 96: 738–742.
Laferriere L. T., Helgeson J. P., Allen C. 1999. Fertile Solanum tuberosum + S. commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum. Theor. Appl. Genet., 98: 1272–1278.
Kemble R.J., Shepard J.F. 1984. Cytoplasmic DNA variation in a potato protoclonal population. Theor. Appl. Genet., 69: 211–216.
Kumar A. 1994. Somaclonal variation. In: Bradshaw J. E., Mackay G. R. (eds.), Potato genetics. CAB International, Cambridge, pp. 197–212.
Mattheij W. M., Eijlander R., de Konig J.R.A., Louwes K.M. 1992. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L. and the wild species S. circaeifolium subsp. circaeifolium Bitter exhibiting resistance to Phytophthora infestans (Mont.) de Bary and Globodera pallida (Stone) Behrens 1. Somatic hybrids. Theor. Appl. Genet., 83: 459–466.
Menke U., Schilde-Rentschler L., Ruoss B., Zanke C., Hemleben V., Ninnemann H. 1996. Somatic hybrids between the cultivated potato Solanum tuberosum L. and the 1EBN wild species Solanum pinnatisectum Dun.: morphological and molecular characterization. Theor. Appl. Genet., 92: 617–626.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant., 15: 473–497.
Naess S.K., Bradeen J.M., Wielgus S.M., Haberlach G.T., McGrath J.M., Helgeson J.P. 2001. Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines. Mol. Gen. Genom., 265: 694–704.
Novy R. G., Helgeson J. P. 1994. Resistance to potato virus Y in somatic hybrids between Solanum etuberosum and S. tuberosum × S. berthaultii hybrid. Theor. Appl. Genet., 89: 783–786.
Rokka V.M., Xu Y.S., Kankila J., Kuusela A., Pulli S., Pehu E. 1994. Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids. Euphytica, 80: 207–217.
Szczerbakowa A., Borkowska M., Wielgat B. 2000. Plant regeneration from the protoplasts of Solanum tuberosum, S. nigrum and S. bulbocastanum. Acta Physiol. Plant., 22: 3–10.
Szczerbakowa A., Maciejewska U., Pawłowski P., Skierski J. S., Wielgat B. 2001. Electrofusion of protoplasts from Solanum tuberosum, S. nigrum and S. bulbocastanum. Acta Physiol. Plant., 23: 1–11.
Thieme R., Darsow U., Gavrilenko T., Dorokhov D., Tiemann H. 1997. Production of somatic hybrids between S. tuberosum L. and late blight resistant Mexican wild potato species. Euphytica, 97: 189–200.
Wielgat B., Wasilewska D. 2001. Somatic hybridization between wild and cultivated Solanum species. Biotechnologia, 2 (53): 9–15 (in Polish).
Wolters A.M.A., Koornneef M., Gilissen L.J.W. 1993. The chloroplast and mitochondrial DNA type are correlated with the nuclear composition of somatic hybrid calli of Solanum tuberosum and Nicotiana plumbaginifolia. Theor. Appl. Genet., 24: 260–267.
Yamada T., Misoo S., Ishii T., Ito Y., Takaoka K., Kamijima O. 1997. Characterization of somatic hybrids between tetraploid Solanum tuberosum L. and dihaploid S. acaule. Breeding Sci., 47: 229–236.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Szczerbakowa, A., Bołtowicz, D. & Wielgat, B. Interspecific somatic hybrids Solanum bulbocastanum (+) S. tuberosum H-8105. Acta Physiol Plant 25, 365–373 (2003). https://doi.org/10.1007/s11738-003-0018-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11738-003-0018-z