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Kinetics of 14C-labelled sucrose, myo-inositol and phosphatidylcholine uptake during induction and differentiation in Brassica napus callus culture


The uptake rate of 14C-labelled sucrose, myo-inositol and PC was studied in callus cultures of two oilseed rape cultivars, characterized by different in vitro regeneration ability. Transfer of calli onto regeneration stimulating medium resulted in changes of examined substances uptake rate, which were depended on tissue morphogenic potential. Non-regenerating calli of both cultivars increased uptake rate of sucrose whereas changes in incorporation of other compounds were under genome control. Significant increase of uptake rate of all tested compounds was observed as result of organogenesis initiation. Such differences, in the responses of organogenic and non-organogenic tissue indicate that this parameter could be useful as marker of organogenesis

A correlation was observed between the rate of sucrose uptake and its concentration in the medium, which suggests an advantage to passive transport through the callus cell membrane. Lack of such correlation in the case of other labels indicates that this processes are selective and under cell control.

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2,4-dichlorophenoxyacetic acid


disintegration per minute


dry weight

GA3 :

gibberellic acid


basal Murashige and Skoog medium (1962)






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Żur, I., Skoczowski, A., Pieńkowski, S. et al. Kinetics of 14C-labelled sucrose, myo-inositol and phosphatidylcholine uptake during induction and differentiation in Brassica napus callus culture. Acta Physiol Plant 24, 11–17 (2002).

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Key words

  • Brassica napus
  • callus culture
  • myo-inositol
  • phosphatidylcholine
  • oilseed rape
  • regeneration ability
  • sucrose
  • uptake rate