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Chemical compositions of exudates from succulent plants induced by methyl jasmonate

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Abstract

Methyl jasmonate (JA-Me) promoted leaf abscission of succulent plant, Crassula lycopodioides, and exudation from stems and leaves after leaf abscission when it was sprayed in whole plants. Major component of the exudate was identified as sucrose based on the results described below.

  1. (1)

    The reaction of the excudate was not positive in the Somogyi-Nelson method but in the phenol-sulfuric acid method.

  2. (2)

    The exudate was hydrolyzed with invertase of Candida utilis, after that showing a positive reaction in the Somogyi-Nelson method.

  3. (3)

    Only glucose and mannose were identified in the exudate after hydrolysis with trifluoroacetic acid using a gas-liquid chromatograph (GLC).

  4. (4)

    A single peak corresponding to the retention time of sucrose was observed using a high performance liquid chromatograph (HPLC).

In addition, potassium and sodium were found in the exudate as major inorganic cations. These results suggest that exudate of Crassula lycopodioides is phloem sap, and JA-Me promotes the excudation due to the inhibition of the synthesis of callose in sieve tubes of succulent plants.

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Authors and Affiliations

  1. College of Integrated Arts & Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, 599-8531, Sakai, Osaka, Japan

    Junichi Ueda, Kensuke Miyamoto, Tomoki Hoshino & Maki Utsunomiya

  2. Research Institute of Pomology and Floriculture, 96-100, Skierniewice, Poland

    Marian Saniewski

  3. Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Fukuoka, Japan

    Hiroshi Okubo

Authors
  1. Junichi Ueda
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  2. Kensuke Miyamoto
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  3. Marian Saniewski
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  4. Tomoki Hoshino
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  5. Maki Utsunomiya
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  6. Hiroshi Okubo
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Ueda, J., Miyamoto, K., Saniewski, M. et al. Chemical compositions of exudates from succulent plants induced by methyl jasmonate. Acta Physiol Plant 24, 187–194 (2002). https://doi.org/10.1007/s11738-002-0010-z

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  • DOI: https://doi.org/10.1007/s11738-002-0010-z

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