Abstract
Thylakoid polypeptides samples from fresh and dark-incubated (0–6 days) leaves were tested for changes in relative abundancies of Lhca polypeptides using a set of polyclonal and monoclonal antibodies in conjunction with immunoblot technique and integrating densitometry. All Lhca polypeptides were found to be more stable within 6 days of dark incubation than bulk chlorophyll itself. On chlorophyll basis the relative abundance of Lhca 1–4 increased after 6 days of dark incubation to 154, 177, 144 and 121 %, respectively, of the values found in fresh leaves.
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Abbreviations
- BSA:
-
bovine serum albumine
- SDS-PAGE:
-
sodium dodecylsulphate polyacrylamide gel electrophoresis
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Jackowski, G., Żelisko, A. Lhca polypeptides are maintained during dark-induced senescence of leaves. Acta Physiol Plant 23, 437–442 (2001). https://doi.org/10.1007/s11738-001-0054-5
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DOI: https://doi.org/10.1007/s11738-001-0054-5