Abstract
Changes in the patterns of gene expression were analyzed in ultraviolet-B (UV-B; 280–320 nm) -treated seedlings of European beech by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) of mRNA. Utilizing 12 different downstream primers and 27 degenerated 10-mer upstream primers 50 induced and 29 repressed cDNA bands were detected. Nineteen bands were subcloned and sequenced. Two clones revealed significant homologies to known database sequences: putative nascent polypeptide-associated complex alpha and 60S ribosomal protein L37. However, only the latter was confirmed to be UV-B-induced by northern blot analysis.
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Abbreviations
- CHS:
-
chalcone synthase
- DDRT-PCR:
-
differential display reverse transcriptase polymerase chain reaction
- EST:
-
expressed sequence tag
- PAL:
-
phenylalanine ammonia-lyase
- PPFR:
-
photosynthetic photon fluence rate
- UV-B:
-
ultraviolet B, 280–320 nm
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Ernst, D., Liegl, I., Kiefer, E. et al. Early changes in mRNA populations in leaves of ultraviolet-B-treated European beech (Fagus sylvatica L.) seedlings. Acta Physiol Plant 23, 343–349 (2001). https://doi.org/10.1007/s11738-001-0042-9
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DOI: https://doi.org/10.1007/s11738-001-0042-9