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Peroxidase involvement in the defense response of red raspberry to Didymella applanata (Niessl/Sacc.)

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Abstract

Peroxidase activity of red raspberry canes was dependent on the cultivar and influenced the subsequent lignification. After inoculation with Didymella applanata, responsible for the spur blight cane disease, the activity of soluble cytoplasmic enzyme increased in the moderately resistant ‘Latham’ and susceptible ‘Malling Promise’, similarly for syringaldazine and guaiacol as hydrogen donors. Systemic induction found in ‘Latham’ was recognized as a symptom of defence mechanism responsible for fungal restriction. Locally enhanced peroxidase activity in the ‘M.Promise’ tissues was related to the local lignification and/or may be associated with the loss of cell integrity caused by pathogen penetration. Pathogen-induced changes of cell wall peroxidases were similar in both cultivars mentioned above. No influence of the infection was found in the high susceptible Zeva cultivar.

Using native-PAGE analysis and horizontal starch electrophoresis of soluble fraction five constitutive acidic isoperoxidases were detected in ‘Latham’ and three in ‘M. Promise’. The infection process was accompanied by the appearance of two new anodic isoforms.

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Authors and Affiliations

  1. Department of Plant Physiology, August Cieszkowski Agricultural University, Wołyńska 35, 60-637, Poznań, Poland

    Monika Kozłowska & Krzysztof Fryder

  2. Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland

    Bogdan Wolko

Authors
  1. Monika Kozłowska
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  2. Krzysztof Fryder
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  3. Bogdan Wolko
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Kozłowska, M., Fryder, K. & Wolko, B. Peroxidase involvement in the defense response of red raspberry to Didymella applanata (Niessl/Sacc.). Acta Physiol Plant 23, 303–310 (2001). https://doi.org/10.1007/s11738-001-0037-6

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  • DOI: https://doi.org/10.1007/s11738-001-0037-6

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