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Effects of sparrow-pecking moxibustion on the temperature and blood flow perfusion of moxibustion point area and uterus and serum AVP, PGF2α, and TXB2 in rats with dysmenorrhea

雀啄灸对痛经大鼠施灸穴区及子宫温度、血流灌注和血清AVP、PGF2α及TXB2的影响

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Abstract

Objective

To observe the effects of sparrow-pecking moxibustion at Shenque (CV8) and Guanyuan (CV4) on the writhing reaction and score, the temperature and blood flow perfusion of moxibustion point area and uterus, the serum levels of arginine vasopressin (AVP), prostaglandin (PG) F2α, and thromboxane (TX) B2 in rats with primary dysmenorrhea (PD) due to cold-dampness stagnation, and to explore the possible mechanism of sparrow-pecking moxibustion in treating PD.

Methods

Thirty-two healthy non-pregnant female Wistar rats were randomly divided into a normal group, a model group, an ibuprofen group, and a sparrow-pecking moxibustion group, with 8 rats in each group. Except for the normal group, the other three groups were subjected to modeling with cold water bath combined with estradiol benzoate and oxytocin injection. Rats in the sparrow-pecking moxibustion group were treated with sparrow-pecking moxibustion at Shenque (CV8) and Guanyuan (CV4) on the 8th day of modeling, 30 min/time, once a day for 3 d; those in the ibuprofen group were treated with 0.8 mL ibuprofen solution (a specification of 125 mg in 10 mL) on the 8th day of modeling, once a day for 3 d; those in the normal group and the model group were given 0.8 mL normal saline, once a day for 3 d. On the 11th day, rats in each group were intraperitoneally injected with oxytocin (2 U/rat), and the writhing incubation period and writhing score in 20 min were observed; the temperature and the blood perfusion of Shenque (CV8), Guanyuan (CV4), and uterus in vivo were detected; the serum levels of AVP, PGF2α, and TXB2 were determined.

Results

The writhing incubation period was significantly longer (P<0.01) and the writhing score was significantly lower (P<0.01) in the sparrow-pecking moxibustion group and the ibuprofen group than in the model group; compared with the ibuprofen group, the writhing incubation period was prolonged (P<0.01) and the writhing score was decreased (P<0.01) in the sparrow-pecking moxibustion group; compared with the normal group, the temperature and the blood perfusion of Shenque (CV8), Guanyuan (CV4), and uterus were significantly decreased, while the serum PGF2α, AVP, and TXB2 levels were significantly increased (P<0.01) in the model group; compared with the model group, the temperature and the blood perfusion of Shenque (CV8), Guanyuan (CV4), and uterus were significantly increased, and the serum levels of PGF2α, AVP, and TXB2 were significantly decreased in the ibuprofen group and the sparrow-pecking moxibustion group (P<0.05 or P<0.01); compared with the ibuprofen group, the temperature and the blood perfusion of Shenque (CV8), Guanyuan (CV4), and uterus were significantly increased (P<0.05), the serum AVP and TXB2 levels were significantly decreased (P<0.05), while the serum PGF2α level had no statistical difference in the sparrow-pecking moxibustion group (P>0.05).

Conclusion

Sparrow-pecking moxibustion had a remarkable analgesic effect on the rats with PD due to cold-dampness stagnation, and the mechanism may be related to the increased temperature and blood perfusion of the moxibustion point area and uterus, as well as the decreased serum PGF2α, AVP, and TXB2 levels.

摘要

目的

观察雀啄灸神阙、关元对寒湿凝滞型原发性痛经(PD)大鼠扭体反应及评分, 施灸穴区及子宫温度值, 子 宫血流灌注量, 大鼠血清精氨酸血管加压素(AVP), 前列腺素(PG) F2α及血栓素(TX)B2含量的影响, 探讨雀啄灸治疗PD的 可能机制。

方法

将32只雌性未孕健康Wistar大鼠采用随机数字表法分为正常组、模型组、布洛芬组及雀啄灸组, 每 组8只。除正常组外, 其余三组采用冷水浴结合苯甲酸雌二醇复合缩宫素注射的方法造模。雀啄灸组大鼠于造模第 8天接受雀啄灸神阙和关元治疗, 每次30 min, 1次/日, 连续3 d; 布洛芬组大鼠于造模第8天接受布洛芬溶液(规格为 10 mL, 125 mg) 0.8 mL灌服治疗, 1次/日, 连续3 d; 正常组与模型组给予0.8 mL 生理盐水灌胃, 1次/日, 连续3 d。第11天, 各组大鼠腹腔注射缩宫素(2 U/只)。观察各组大鼠20 min内扭体潜伏期及扭体评分, 观测大鼠神阙、关元穴区及在体 子宫的温度值及血流灌注量, 检测大鼠血清中AVP、PGF2α及TXB2的含量。

结果

雀啄灸组及布洛芬组扭体潜伏期较模 型组明显延长(P<0.01), 扭体评分显著降低(P<0.01); 与布洛芬组比较, 雀啄灸组扭体潜伏期延长(P<0.01), 扭体评分降 低(P<0.01); 与正常组比较, 模型组大鼠神阙、关元穴区及子宫温度值, 以及子宫血流灌注量明显降低, 血清PGF2α、AVP 及TXB2含量明显升高(P<0.01); 与模型组比较, 布洛芬组、雀啄灸组大鼠神阙、关元穴区及子宫温度值, 以及子宫血流 灌注量均明显升高, 血清PGF2α、AVP及TXB2的含量明显降低(P<0.05或P<0.01);与布洛芬组比较, 雀啄灸组大鼠神阙、 关元穴区及子宫温度值, 以及子宫血流灌注量均明显升高(P<0.05), 血清AVP及TXB2含量明显降低(P<0.05), 血清PGF2α 含量无统计学差异(P>0.05)。

结论

雀啄灸对寒湿凝滞型原发性痛经镇痛效果显著, 其机制可能与提高灸治穴区、子 宫温度值及子宫血流灌注量, 降低血清PGF2α、AVP及TXB2含量有关。

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Acknowledgments

This work was supported by the Science and Technology Research Project of Colleges and Universities in Hebei Province (河北省高等学校科学技术研究项目, No. ZD2019099).

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Authors and Affiliations

  1. Hebei University of Chinese Medicine, Shijiazhuang, 050091, China

    Chenxi Zhou  (周晨曦), Xinhua Li  (李新华), Xiangyun Meng  (孟祥云), Chao Liu  (刘超), Xuanping Zhang  (张选平), Yanqiu Sun  (孙艳秋), Xuan Zhang  (张璇), Yulei Liang  (梁玉磊) & Zhiguo Zhao  (赵志国)

  2. Key Research Office of Hebei Provincial Needling and Moxibustion Techniques Effect Specificity, Shijiazhuang, 050091, China

    Xinhua Li  (李新华), Xuanping Zhang  (张选平), Yulei Liang  (梁玉磊) & Zhiguo Zhao  (赵志国)

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  1. Chenxi Zhou  (周晨曦)
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Correspondence to Xinhua Li  (李新华) or Zhiguo Zhao  (赵志国).

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The treatment of animals conformed to the ethical criteria in this experiment.

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First Author: ZHOU Chenxi, master degree candidate

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Zhou, C., Li, X., Meng, X. et al. Effects of sparrow-pecking moxibustion on the temperature and blood flow perfusion of moxibustion point area and uterus and serum AVP, PGF2α, and TXB2 in rats with dysmenorrhea. J. Acupunct. Tuina. Sci. 21, 265–272 (2023). https://doi.org/10.1007/s11726-023-1384-2

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  • DOI: https://doi.org/10.1007/s11726-023-1384-2

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