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Effect of scraping therapy on Th1/Th2 balance in rats with lumbar disc herniation

刮痧对腰椎间盘突出症大鼠血清Th1/Th2免疫平衡的影响

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Abstract

Objective

To observe the effect of scraping therapy (ST) on the immune balance of serum helper T (Th) 1/Th2 cells in autologous nucleus pulposus transplantation non-compressive lumbar disc herniation (LDH) model rats, and explore the immune mechanism of ST in reducing LDH pain.

Methods

Seventy-two male Sprague-Dawley (SD) rats were randomly divided into a model group, a sham operation group and a ST group, with 24 rats in each group. Autologous nucleus pulposus transplantation non-compressive LDH models were established in the model group and ST group, while the rats in the sham operation group underwent sham operation without model establishment. On the fifth day after the model was successfully prepared, rats in the ST group received ST, once every other day, 3 times as a course for a total of 3 courses. Six rats in each group were randomly selected to observe their pain thresholds, and peripheral blood of the rats was collected before the first scraping treatment and at the end of the first, second, and third courses of treatment. The serum was isolated and enzyme-linked immunosorbent assay (ELISA) was applied for the detection of rat serum interferon (IFN)-γ, interleukin (IL)-4 and IL-10.

Results

Compared with the model group, the pain threshold in LDH rats in the ST group increased (P<0.05), the serum IFN-γ level was significantly reduced (P<0.05), but the levels of IL-4 and IL-10 did not change significantly (both P>0.05). At the end of the second and third courses of treatment, the IFN-γ/IL-4 ratio was negatively correlated with the pain threshold in the rats, and the IFN-γ/IL-4 ratio was significantly reduced in the ST group (P<0.01).

Conclusion

ST can help suppress the Th1 immunity in LDH rats triggered by the autologous nucleus pulposus, restore the immune balance of Th1/Th2, and reduce the pain of LDH.

摘要

目的

观察刮痧对自体髓核移植非压迫性腰椎间盘突出症(LDH)模型大鼠血清辅助T(Th)1/Th2细胞免疫平 衡的影响, 探究刮痧降低LDH疼痛的免疫作用机制.

方法

将72只雄性Sprague-Dawley (SD)大鼠随机分为模型组、 假手术组和刮痧组, 每组24只。模型组与刮痧组大鼠建立自体髓核移植非压迫性LDH模型, 假手术组只做假手术 处理。模型组与假手术组不予治疗, 刮痧组于造模后第5天开始干预治疗, 隔日刮1次, 3次为1个疗程, 共3个疗程。 分别于造模后、刮痧前、第1疗程结束时、第2疗程结束时和第3疗程结束时, 每组随机取6只大鼠, 观察其痛阈, 并收集大鼠外周血。采用酶联免疫吸附测定法(ELISA)检测大鼠血清干扰素(IFN)-γ、白介素(IL)-4和IL-10.

结果

与 模型组比较, 刮痧能升高LDH大鼠痛阈值(P<0.05), 显著降低血清IFN-γ水平(P<0.05), 对IL-4和IL-10水平无显著影 响(均P>0.05)。第2、3疗程结束时, IFN-γ/IL-4比值水平与大鼠痛阈值呈负相关, 刮痧组IFN-γ/IL-4比值明显降低 (P<0.01).

结论

刮痧能抑制自体髓核引起的LDH大鼠Th1免疫, 使机体Th1/Th2免疫失衡恢复到正常状态, 并减 轻其疼痛.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (国家自然科学基金项目, No. 81473791); Advantage Discipline Construction Project of Nursing in Jiangsu Universities (江苏高校护理学优势学科建设工程资助项目, No. 2019YSHL028); Undergraduate Practice Innovation Training Program of Nanjing University of Traditional Chinese Medicine (南京 中医药大学大学生实践创新训练计划项目, No. 201810315121).

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Authors and Affiliations

  1. School of Nursing, Nanjing University of Traditional Chinese Medicine, Nanjing, 210000, China

    Min Yang  (杨敏) & Gui-hua Xu  (徐桂华)

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  1. Min Yang  (杨敏)
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  2. Gui-hua Xu  (徐桂华)
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Correspondence to Gui-hua Xu  (徐桂华).

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The authors declare that there is no potential conflict o: interest in this article.

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The treatment of animals conformed to the ethical criteria in this experiment.

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Yang, M., Xu, Gh. Effect of scraping therapy on Th1/Th2 balance in rats with lumbar disc herniation. J. Acupunct. Tuina. Sci. 18, 330–336 (2020). https://doi.org/10.1007/s11726-020-1198-4

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  • DOI: https://doi.org/10.1007/s11726-020-1198-4

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