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Effect of electroacupuncture on anti-Mullerian hormone expression in rats with polycystic ovarian syndrome


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To observe whether the effect of electroacupuncture (EA) on improving sex hormone disorders and follicle development is by decreasing the expression of anti-Mullerian hormone (AMH) in rats with experimental polycystic ovarian syndrome (PCOS).


Forty rats were randomly divided into four groups, a normal group (NG), a model group (MG), an EA at acupoints group (EAAG), and an EA at non-acupoints group (EANAG), with 10 rats in each group. The rats in the EAAG and EANAG were intervened by EA treatment for consecutive 14 d. Zhongji (CV 3) and Guanyuan (CV 4) were selected as the acupoints in the EAAG, and the tip of the tail and 1 cm up from the tail tip were selected as the non-acupoints in the EANAG. After treatment, the histomorphological changes of the ovary, the levels of aromatase P450 (P450arom), testosterone and estradiol in the ovarian tissues, and the expressions of follicle stimulating hormone (FSH) and AMH were observed.


After treatment, compared with the MG and EANAG, the expression of AMH decreased (P<0.05), the levels of P450arom and estradiol increased significantly, and the level of testosterone decreased significantly (all P<0.01) in the EAAG. Additionally, several normal follicles were present and the number of cystically dilated follicles decreased in the EAAG. Compared with the MG and EANAG, the EAAG obviously had more follicular granulosa cells.


EA can down-regulate the abnormally increased expression of AMH to improve sex hormone disorders and follicle development in PCOS rats.





将 40 只大鼠随机分为正常组、 模型组、 电针经穴组和电针非经穴组, 每组 10 只。 电针经穴组和电针非经穴组大鼠连续治疗 14 d。 电针经穴组大鼠取中极和关元; 电针非经穴组取尾尖和尾尖上 1 cm 处。 观察治疗后卵巢组织形态学变化, P450 芳香化酶、 睾酮和雌二醇水平以及促卵泡生成素(FSH)和AMH的表达。


同模型组和电针非经穴组比较, 电针经穴组 AMH 表达降低(P<0.05), P450 芳香化酶和雌二 醇水平显著升高, 睾酮水平显著降低(均P<0.01)。 此外, 电针经穴组存在数个正常卵泡, 囊性扩张卵泡减少, 卵泡颗粒细胞数明显多于模型组和电针非经穴组。


电针可下调 PCOS 大鼠 AMH 的异常过度表达, 从而改善性激素紊乱和卵泡发育障碍。

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This work was supported by National Basic Research Program of China (973 Program, 国家重点基础研究发展 计划项目, No. 2015CB554500); Research Project of Autonomous Selection of China Academy of Traditional Chinese Medicine (中国中医科学院自主选题研究项目, No. ZZKF08002); Project of Shanghai Municipal Commission of Health and Family Planning (上海市卫生 和计划生育委员会科研课题, No. 201840307); Shanghai Academic Community of Leading Talents in Chinese Medicine ( 上海市中医药领军人才项目, No. ZYSNXD-RC-LJRC). We are grateful for the strong support of JRDUN Biotechnology (Shanghai) Co., Ltd.

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Correspondence to Yin Shi  (施茵) or Liang Li  (李亮).

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Zhou, J., Sun, Y., Sun, J. et al. Effect of electroacupuncture on anti-Mullerian hormone expression in rats with polycystic ovarian syndrome. J. Acupunct. Tuina. Sci. 17, 80–88 (2019).

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