Effect of reward alteration following acupuncture for morphine withdrawal rats on neuronal discharges in the ventral medial prefrontal cortex
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To observe the effect of reward alteration following acupuncture for morphine withdrawal rats on the behavior and neuronal discharges in the medial prefrontal cortex (mPFC).
The Sprague-Dawley (SD) rats were randomly allocated into a model group, a confinement group, an electroacupuncture (EA) group, and a control group. Rats with morphine addiction were made by intraperitoneal injection of naloxone (same dose injection of saline for rats in the control group), followed by a 2-week morphine withdrawal. Acupuncture and confinement were completed during the morphine withdrawal period. Upon withdrawal, the rats received conditioned place preference (CPP) training and open field test. The multi-channel neural signal processor was used in the electrophysiological experiment to measure the neuronal discharges in different subareas of prefrontal cortex in CPP box and aversion box.
Rats in the model group and the confinement group spent longer period of time in CPP box than those in the EA group and the control group (all P<0.01); there was no statistically significant difference between the EA group and the control group. The total distances of movement by rats in the model group and the confinement group were longer than those in the EA group and the control group (all P<0.01). The mPFC neuronal discharge frequencies were compared between morphine preference box and aversion box. The mPFC neuronal discharge frequencies in the model group and the confinement group were higher than those in the EA group and the control group (all P<0.05); there was no statistically significant difference between the EA group and the control group.
Acupuncture can effectively interfere with the reward alteration following morphine withdrawal, possibly because of its involvement with the mPFC neuronal discharges.
KeywordsAcupuncture Therapy Substance Withdrawal Syndrome Morphine Dependence Behavior Reward Ventral Medial Prefrontal Cortex Rats
将SD大鼠随机分为模型组、捆绑组、电针组、对照组。建立大鼠吗啡成瘾动物模型(对照组注射等量生理盐水), 经纳洛酮腹腔注射确认造模成功, 所有大鼠进入两星期吗啡戒断期, 针刺与捆绑在吗啡戒断期完成。戒断后进行 条件性位置偏爱训练和旷场测试。电生理实验使用多通道神经元放电同步记录系统, 测定各组吗啡戒断后大鼠在 条件性位置偏爱箱和非偏爱箱中不同的前额皮层放电活动。
模型组、捆绑组大鼠在偏爱箱时间高于电针 组和对照组(均P<0.01), 电针组和对照组之间无显著性差异。模型组和捆绑组大鼠在旷场箱中的总路程高于电针 组和对照组(均P<0.01)。大鼠mPFC神经元放电频率在吗啡偏爱箱与非偏爱箱比值, 模型组和捆绑组高于电针组和 对照组(均P<0.05), 电针组和对照组之间无显著性差异。
针刺有效干预大鼠吗啡戒断后奖赏改变, 可能涉 及mPFC神经元放电活动。
关键词针刺疗法 物质禁断综合征 吗啡依赖 行为 奖赏 腹侧前额叶皮层 大鼠
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This work was supported by National Natural Science Foundation of China (国家自然科学基金项目, No. 81373753); Natural Science Foundation of Shanghai (上海 市自然科学基金项目, No. 13ZR1441900); the Budgeted Scientific Research Project of Shanghai University of Traditional Chinese Medicine (上海中医药大学预算内项 目, No. 2013JW16).
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