Effect of acupuncture at points selected from different regions on SCF-kit signaling pathway in diabetic gastroparesis rats
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To observe the effect of acupuncture at points selected from different regions on the positive expression of interstitial cells of Cajal (ICC) and the stem cell factor (SCF) in gastric antrum tissues in diabetic gastroparesis (DGP) rats, and to explore the influence of region-based point selection on the acupoint combination efficacy.
Sixty Sprague-Dawley (SD) rats were randomly divided into a normal group (group A), a model group (group B), a group of Zusanli (ST 36) plus Zhongwan (CV 12) (group C), a group of Zusanli (ST 36) plus Neiguan (PC 6) (group D), and a group of Zusanli (ST 36) plus non-meridian non-acupoint points (group E), based on the random number table (n=12). DGP rat model was established by single intraperitoneal injection of 2% streptozotocin and common diet. After successful modeling, the rats were treated once a day for 4 weeks. Positive ICC and SCF expressions were measured by immunohistochemistry.
Compared with group A, the gastrointestinal propulsion rate of group B showed a statistically significant decrease (P<0.05). Compared with group B, the gastrointestinal propulsion rate and the expression of ICC in the gastric antrum were significantly higher in group C, group D and group E, and the between-group differences were statistically significant (P<0.05); the expression of C-kit protein in group C was statistically significantly higher than that in group D and group E (P<0.05). The expression of SCF protein was significantly increased in group C than in group B, and the difference was statistically significant (P<0.05).
Acupuncture can improve the symptoms of delayed gastric emptying in DGP model rats, and regulate the expression of ICC and SCF in gastric antrum tissues. The effect of Zusanli (ST 36) plus Zhongwan (CV 12) in the gastric region is superior to that of the Zusanli (ST 36) plus distal Neiguan (PC 6) or non-meridian non-acupoint point, indicating that region-based point selection is the key factor affecting the effect of acupoint combination.
KeywordsAcupuncture Therapy Specificity of Acupoints Point Selection Interstitial Cells of Cajal Diabetes Complications Gastroparesis Rats
观察不同按部选穴针刺对糖尿病胃轻瘫(DGP)大鼠胃窦组织Cajal 间质细胞(ICC)阳性表达及干细胞 因子(SCF)的影响, 探讨按部选穴对腧穴配伍效应的影响。
将60 只Sprague-Dawley(SD)大鼠根据随机数字表 随机分为正常组、模型组、足三里加中脘组、足三里加内关组、足三里加非经非穴组, 每组12 只。采用单次腹 腔注射2%链脲佐菌素配合普通饲料饮食建立DGP 大鼠模型。造模成功后, 每日治疗1 次, 连续治疗4 星期。用 免疫组织化学法测定ICC 阳性及SCF 表达。
与正常组比较, 模型组胃肠推进率明显减低, 组间差异有统计 学意义(P<0.05)。与模型组比较, 足三里加中脘组、足三里加内关组、足三里加非经非穴组胃肠推进率、胃窦组 织ICC 表达显著升高, 组间差异有统计学意义(P<0.05); 足三里加中脘组C-kit 蛋白表达显著高于足三里加内关 组、足三里加非经非穴组, 组间差异具有统计学意义(P<0.05); 与模型组比较, 足三里加中脘组SCF 蛋白表达明 显升高, 组间差异有统计学意义(P<0.05)。
针刺可改善DGP 模型大鼠胃排空迟缓症状, 调节胃窦组织ICC 及SCF 的表达, 足三里配合胃脘部的中脘穴的效果显著优于足三里配伍远端的内关穴或非经非穴点, 表明按部选 穴是影响腧穴配伍效应的关键因素。
关键词针刺疗法 穴位特异性 选穴 Cajal 间质细胞 糖尿病并发症 胃轻瘫 大鼠
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This work was supported by National Basic Research Program of China (973 Program, 国家重点基础研究发展 计划, No. 2014CB543102).
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