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Study on the effect of moxibustion in treating rhreumatoid arthritis rats and its mechanism

艾灸对类风湿关节炎大鼠的治疗作用及其机制探讨

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摘要

目的

观察艾灸对类风湿关节炎大鼠的治疗作用及其机制。

方法

将100 只雄性Wistar 大鼠随机分为5 组。 每组20 只, 除正常组外, 其余4 组大鼠制备类风湿关节炎模型。 造模成功后, 正常组和模型组不予任何治疗。 艾灸组予艾条悬灸; 香烟组予香烟悬灸, 艾油组予艾叶精油穴位涂抹。 连续治疗15 d 后, 比较各组大鼠体重、 趾围、 胸腺指数及脾指数变化, 免疫组化方法检测大鼠关节滑膜中Fas/FasL 蛋白的表达。

结果

艾灸组大鼠体重增加, 关节肿胀减轻, 胸腺指数提高、 脾指数下降, 关节滑膜中Fas/FasL 蛋白的表达增加。 在改善上述指标方面, 艾灸组、 香烟组较艾油组明显; 艾灸组在Fas/FasL 蛋白表达方面优于香烟组。

结论

艾灸治疗能显著改善大鼠类风湿关节炎症状, 调节关节滑膜中Fas/FasL 蛋白的表达, 诱导滑膜细胞凋亡, 这可能是艾灸发挥温通作用的有效机制之一。

Abstract

Objective

To investigate the therapeutic efficacy of moxibustion in treating rheumatoid arthritis (RA) and its mechanism.

Methods

A hundred male Wistar rats were randomized into 5 groups, 20 in each group. RA models were developed in all groups except for the normal group. When the models were successfully prepared, the rats in the normal group and the model group didn’t receive any treatment. Those in the moxibustion group received suspended moxibustion by moxa stick, those in the cigarette group received fumigation by cigarette; while those in the moxa oil group were treated by applying moxa essential oil to acupoints. After consecutive 15-day treatment, body weight, paw circumference, thymus and spleen indexes were compared among the five groups, and the expression of Fas/FasL protein in synovium of rat’s joint was detected by using immunohistochemical (IHC) method.

Results

After treatment, the moxibustion group had body weight increased, joint swelling relieved, thymus index enhanced, spleen index dropped, and the expression of Fas/FasL protein raised. Regarding the above indexes, the moxibustion group and the cigarette group showed more significant improvements than the moxa oil group; the moxibustion group had more significant effect on the expression of Fas/FasL than the cigarette group.

Conclusion

Moxibustion obviously improves the symptoms of RA rats, regulates the expression of Fas/FasL protein in synovium of rat’s joint, and induces apoptosis of synovial cells, which maybe one of the mechanisms of moxibustion in achieving the warming and unblocking effect.

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Authors and Affiliations

  1. Anhui University of Traditional Chinese Medicine, Hefei, 230038, China

    Chuan-yu Peng  (彭传玉), Lei Luo  (罗磊), Ling Hu  (胡玲), Zi-jian Wu  (吴子建), Rong-lin Cai  (蔡荣林), Feng Hao  (郝峰) & Wu-bin Hu  (胡吴斌)

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  1. Chuan-yu Peng  (彭传玉)
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  2. Lei Luo  (罗磊)
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  3. Ling Hu  (胡玲)
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  4. Zi-jian Wu  (吴子建)
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  5. Rong-lin Cai  (蔡荣林)
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  6. Feng Hao  (郝峰)
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  7. Wu-bin Hu  (胡吴斌)
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Correspondence to Ling Hu  (胡玲).

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Author: Peng Chuan-yu, master degree candidate of year 2009

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Peng, Cy., Luo, L., Hu, L. et al. Study on the effect of moxibustion in treating rhreumatoid arthritis rats and its mechanism. J. Acupunct. Tuina. Sci. 10, 336–341 (2012). https://doi.org/10.1007/s11726-012-0632-7

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  • DOI: https://doi.org/10.1007/s11726-012-0632-7

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