摘要
目的
观察艾灸对克罗恩病(Crohn Disease, CD)肠纤维化大鼠结肠转化生长因子-β (Transforming Growth Factor-β, TGF-β)蛋白、 Smad4 蛋白及mRNA 表达的影响, 从TGF-β/Smads 信号途径探讨针灸治疗CD 肠纤维化的作用机制。
方法
采用雄性清洁级SD 大鼠建立CD 肠纤维化模型, 应用随机的方法, 将大鼠分为正常组、 模型组、 温和灸组、 电针组、 隔药灸组。 正常组和模型组不予任何治疗; 温和灸组、 电针组、 隔药灸组均取天枢、 气海穴, 分别施以温和灸、 电针、 隔药灸治疗。 治疗结束后, 采用免疫组织化学法(Immunohistochemistry, IHC)检测各组大鼠结肠TGF-β 与Smad4 蛋白的表达; 采用荧光定量聚合酶链反应法(Fluorescence Quantitative Polymerase Chain Reaction, FQ-PCR)检测结肠Smad4 mRNA 的表达。
结果
与正常组比较, 模型组大鼠结肠TGF-β 蛋白、 Smad4 蛋白及mRNA 表达增加(P<0.01)。 经温和灸、 电针、 隔药灸治疗后, 与模型组比较, TGF-β 蛋白、 Smad4 蛋白及mRNA 表达均有不同程度的降低(P<0.01 或P<0.05)。
结论
CD 肠纤维化大鼠结肠TGF-β 蛋白、 Smad4 蛋白及mRNA 表达显著增多, 温和灸、 电针、 隔药灸天枢和气海穴均可下调CD 肠纤维化大鼠结肠TGF-β 蛋白、 Smad4 蛋白及mRNA 的异常表达。
Abstract
Objective
To observe the effect of moxibustion on the expressions of transforming growth factor-β (TGF-β) protein, and Smad4 protein and mRNA in rats with intestinal fibrosis in Crohn disease (CD), and to discuss the action mechanism of acupuncture-moxibustion in treating intestinal fibrosis in CD from the perspective of TGF-β/Smads signal pathway.
Methods
A CD intestinal fibrosis model was developed by using male SD rats of clean grade. The rats were randomized into a normal group, a model group, a mild moxibustion group, an electroacupuncture (EA) group, and a herb-partitioned moxibustion group. The rats in the normal and the model groups did not receive any interventions. Tianshu (ST 25) and Qihai (CV 6) were selected for the rats in the mild moxibustion group, the EA group, and the herb-partitioned moxibustion group. Mild moxibustion, EA, and herb-partitioned moxibustion were given respectively. After treatment, the expressions of TGF-β and Smad4 proteins in rat’s colon were detected by using immunohistochemistry (IHC); the expression of Smad4 mRNA was determined by using fluorescence quantitative polymerase chain reaction (FQ-PCR).
Results
Compared with the normal group, the expressions of TGF-β protein, Smad4 protein and Smad4 mRNA significantly increased in the model group (P<0.01). Compared with the model group, the expressions of TGF-β protein, Smad4 protein, and Smad4 mRNA dropped markedly (P<0.01 or P<0.05) after treatments of mild moxibustion, EA, and herb-partitioned moxibustion.
Conclusion
The expressions of TGF-β protein, Smad4 protein and Smad4 mRNA significantly increase in CD rats with intestinal fibrosis; while mild moxibustion, EA and herb-partitioned moxibustion at Tianshu (ST 25) and Qihai (CV 6) all can down-regulate the abnormal expressions.
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References
Massague J, Blain SW, Lo RS. TGFbeta signaling in growth control, cancer, and heritable disorders. Cell, 2000, 103(2): 295–309.
Burke JP, Mulsow JJ, O’Keane C, Docherty NG, Watson RW, O’Connell PR. Fibrogenesis in Crohn’s disease. Am J Gastroenterol, 2007, 102(2): 439–448.
Rutgeerts P, Geboes K, Vantrappen G, Beyls J, Kerremans R, Hiele M. Predictability of the postoperative course of Crohn’s disease. Gastroenterology, 1990, 99(4): 956–963.
Rutgeerts P, Van Assche G, Vermeire S, D’Haens G, Baert F, Noman M, Aerden I, De Hertogh G, Geboes K, Hiele M, D’Hoore A, Penninckx F. Ornidazole for prophylaxis of postoperative Crohn’s disease recurrence: a randomized, double-blind, placebo-controlled trial. Gastroenterology, 2005, 128(4): 856–861.
Liu HR, Ma XP, Zhao TP, Wu HG. Regulation of acupuncture-moxibustion on collagen and TGF-β mRNA in colon of Crohn’s disease rats. Anhui Zhongyi Xueyuan Xuebao, 2005, 24(4): 25–30.
Ma XP, An CP, Wu HG, Liu HR, Shi Z, Yang L, Yang S. Herbs-partitioned moxibustion and elecroacupuncture effects on transforming growth factor-beta 1, connective tissue growth factor, collagen type I and fibronectin expression in the colon of Crohn’s disease rats. Zhongguo Zuzhi Gongcheng Yu Linchuang Kangfu, 2008, 12(20): 3853–3858.
Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, Wallace JL. Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology, 1989, 96(3): 795–803.
Kitamura Y. Heterogeneity of mast cells and phenotypic change between subpopulations. Ann Rev Immunol, 1989, 7: 59–76.
Lindemann S, Tolley ND, Dixon DA, McIntyre TM, Prescott SM, Zimmerman GA, Weyrich AS. Activated platelets mediate inflammatory signaling by regulated interleukin 1 beta synthesis. J Cell Biol, 2001, 154 (3): 485–490.
Rojkind M, Ponce-Noyola P. The extracellular matrix of the liver. Coll Relat Res, 1982, 2(2): 151–175.
Inagaki Y, Truter S, Greenwel P, Rojkind M, Unoura M, Kobayashi K, Ramirez F. Regulation of the alpha 2(1) collagen gene transcription in fat-storing cells derived from a cirrhotic liver. Hepatology, 1995, 22(2): 573–579.
Lawrance IC, Maxwell L, Doe W. Inflammation location, but not type, determines the increase in TGF-beta1 and IGF-1 expression and collagen deposition in IBD intestine. Inflamm Bowel Dis, 2001, 7(1): 16–26.
Gao CF, Lu LG. Basics and Clinics of Fibrosis Diseases. Shanghai: Shanghai Scientific and Technical Publishers, 2004: 94–95.
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Wu, Hg., Zhao, C., Wu, Ly. et al. Effect of moxibustion on expressions of TGF-β and Smad4 in colonic mucosa of rats with intestinal fibrosis in Crohn disease. J. Acupunct. Tuina. Sci. 10, 331–335 (2012). https://doi.org/10.1007/s11726-012-0631-8
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DOI: https://doi.org/10.1007/s11726-012-0631-8