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Effect of moxibustion on expression of HSP70 and apoptosisrelated factors in rats with acute gastric mucosal damage

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摘要

目的

观察艾灸预处理对急性胃黏膜损伤大鼠胃黏膜细胞凋亡相关因子的影响, 探讨艾灸诱导HSP70 与上述因子的关系, 从细胞凋亡线粒体信号转导途径探讨艾灸预处理保护胃黏膜损伤的机制。

方法

将32 只Wister大鼠随机分为4 组, 即捆绑组、 模型组、 艾灸穴位组、 艾灸非穴组。 艾灸穴位组和艾灸非穴组大鼠艾灸预处理8 d, 捆绑组和模型组大鼠只捆绑, 不艾灸。 除捆绑组外, 其余各组制备大鼠急性胃黏膜损伤模型。 采用Western-blot方法检测大鼠胃黏膜细胞色素C (Cyt-c) 的表达, 免疫组织化学方法检测胃黏膜HSP70, 胃黏膜细胞凋亡, Apaf-1, Caspase-9, Caspase-3, Bcl-2 和Bax的表达。

结果

与捆绑组相比, 模型组大鼠胃黏膜HSP70 表达, 胃黏膜细胞凋亡指数, 胞浆Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bcl-2 和Bax含量明显增加 (P<0.05 或P<0.01)。 与模型组相比, 艾灸穴位组大鼠胃黏膜HSP70 表达和Bcl-2 明显增加 (P<0.01), 而细胞凋亡指数, 胞浆Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bax均显著降低 (P<0.01)。 与艾灸穴位组相比, 艾灸非穴组大鼠胃黏膜HSP70 表达和Bcl-2 显著降低 (P<0.01), 而凋亡指数, Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bax显著升高(P<0.05 或P<0.01)。

结论

艾灸通过上调大鼠胃黏膜细胞HSP70表达, 并影响细胞凋亡线粒体信号转导途径相关因子Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bcl-2 和Bax, 由此抑制胃黏膜细胞凋亡, 达到保护胃黏膜损伤的作用。

Abstract

Objective

To observe the effect of preventively used moxibustion on the expression of apoptosis-related factors in rats with acute gastric mucosal damage (GMD), and analyze the relationship between those factors and heat shock protein 70 (HSP70), for discovering the mechanism of moxibustion for protecting gastric mucosa from the aspect of mitochondria signal transduction of apoptosis.

Methods

Thirty-two Wistar rats were randomly divided into binding, model, acupoint-moxibustion and non-acupoint-moxibustion groups, 8 in each group. Moxibustion was applied preventively to acupoint-moxibustion and non-acupointmoxibustion groups respectively for 8 d. Rats in binding and model groups were served with restraint without moxibustion. The GMD models were induced in groups except for A group, and the expression of cytochrome c (Cyt-c) in gastric mucosa was measured by Western-blot analysis, the expression of HSP70, cell apoptosis index (AI), apoptotic protease activating factor 1 (Apaf-1), Caspase-9, Caspase-3, Bcl-2 and Bax in gastric mucosal cells were studied by immunohistochemical detection.

Results

Compared with binding group, the expressions of HSP70, AI, Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bcl-2 and Bax of gastric mucosa in model group were significantly higher (P<0.05 or P<0.01). Compared with model group, the expressions of HSP70 and Bcl-2 were significantly higher (P<0.01), while AI, Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bax were significantly lower (P<0.01) in acupoint-moxibustion group. Compared with the acupoint-moxibustion group, the expressions of HSP70 and Bcl-2 were significantly lower (P<0.01), while AI, Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bax were significantly higher (P<0.05 or P<0.01) in non-acupoint-moxibustion group.

Conclusion

Moxibustion inhibits the apoptosis of rats’ gastric mucosal cells and protect them from damage, through up-regulating the expression of HSP70, and modulating the expressions of Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bcl-2 and Bax which were related to the mitochondria signal transduction of apoptosis.

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Authors and Affiliations

  1. College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine, Changsha, 410007, P. R. China

    Shou-xiang Yi  (易受乡), Jie Yu  (郁洁), Xiao-rong Chang  (常小荣), Yan Peng  (彭艳), Jie Yan  (严洁) & Ren-da Yang  (阳仁达)

  2. Shanghai Research Institute of Acupuncture and Meridian, Shanghai, 200030, P. R. China

    Huan-gan Wu  (吴焕淦)

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  1. Shou-xiang Yi  (易受乡)
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  2. Jie Yu  (郁洁)
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  3. Xiao-rong Chang  (常小荣)
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  4. Yan Peng  (彭艳)
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  5. Huan-gan Wu  (吴焕淦)
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  6. Jie Yan  (严洁)
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Additional information

Fund Items: National Basic Research Program of China (973 Program, 2009CB522904); National Natural Science Funds (30772707)

Author:YI Shou-xiang (1946-), female, professor

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Yi, Sx., Yu, J., Chang, Xr. et al. Effect of moxibustion on expression of HSP70 and apoptosisrelated factors in rats with acute gastric mucosal damage. J. Acupunct. Tuina. Sci. 8, 5–11 (2010). https://doi.org/10.1007/s11726-010-0005-z

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  • DOI: https://doi.org/10.1007/s11726-010-0005-z

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