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Effects of acupuncture pretreatment on ischemic cardiac muscle cell apoptosis and gene expression in ischemia-reperfusion rats

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摘要

目的

针灸预处理对缺血心肌具有保护作用。 通过观察针刺预处理对心肌缺血再灌注损伤大鼠心肌细胞凋亡及HSP70mRNA表达的影响, 探讨针刺预处理的心肌保护机制。

方法

64 只Wistar大鼠随机分为8 组, 即正常对照组, 假手术组, 缺血再灌注组, 缺血预处理组, 手捻针预处理日1 次组, 电针预处理日1 次组, 手捻针预处理日2 次组, 电针预处理日2 次组。 建立大鼠心肌缺血再灌注模型, 采用原位杂交法测定心肌HSP70mRNA的表达, TUNEL法检测细胞凋亡。

结果

与正常对照组、 假手术组比较, 缺血再灌注组细胞凋亡增加, HSP70 mRNA表达增加; 与缺血再灌注组比较, 针刺预处理使心肌细胞凋亡减少、 HSP70mRNA表达增加, 且针刺预处理日2 次组作用强于针刺预处理日1 次组和缺血预处理组。

结论

针刺预处理能够抑制心肌缺血再灌注损伤大鼠心肌细胞凋亡, 上调心肌HSP70mRNA的表达。 针刺预处理每日2 次的作用强于针刺预处理每日1 次。

Abstract

Objective

To investigate the protective effects of acupuncture pretreatment on ischemic myocardium, the protective mechanism of acupuncture pretreatment on ischemic myocardium was explored by observing the cardiac muscle cell apoptosis and the expression of HSP70 mRNA of ischemia-reperfusion injury rats treated with acupuncture pretreatment.

Methods

Sixty-four Wistar rats were randomly divided into eight groups: control group, sham surgery group, ischemia-reperfusion group, ischemia pretreatment group, manual acupuncture pretreatment group (once a day), electroacupuncture pretreatment group (once a day), manual acupuncture pretreatment group (twice a day), and electroacupuncture pretreatment group (twice a day). The reperfusion model of rat myocardial ischemia was made. Expression of HSP70 mRNA was assayed by in situ hybridization, and cell apoptosis by TUNEL.

Results

Compared with those in the control group and the sham surgery group, the apoptosis and the expression of HSP70 mRNA were increased in the ischemia-reperfusion group. Compared with those in the ischemia-reperfusion group, the cardiac muscle cell apoptosis was decreased and the HSP70 mRNA was increased in the rats treated with acupuncture pretreatment; meanwhile, acupuncture pretreatment twice a day had stronger effects than acupuncture pretreatment once a day and ischemia pretreatment.

Conclusion

Acupuncture pretreatment can inhibit the cardiac muscle cell apoptosis, and up-regulate the expression of HSP70 mRNA in ischemia-reperfusion rats. Acupuncture pretreatment twice a day has stronger effects than pretreatment once a day.

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Authors and Affiliations

  1. Guanlan People’s Hospital of Baoan District, Shenzhen, Guangdong, 518110, P. R. China

    Yu-hui Zhao  (赵宇辉)

  2. Institute of Acupuncture and Moxibustion, Heilongjiang University of TCM, Haerbin, 150040, P. R. China

    Zhong-ren Sun  (孙忠人)

Authors
  1. Yu-hui Zhao  (赵宇辉)
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  2. Zhong-ren Sun  (孙忠人)
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Additional information

Author: ZHAO Yu-hui (1978–), male, PhD, the dean of rehabilitation department

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Zhao, Yh., Sun, Zr. Effects of acupuncture pretreatment on ischemic cardiac muscle cell apoptosis and gene expression in ischemia-reperfusion rats. J. Acupunct. Tuina. Sci. 7, 71–74 (2009). https://doi.org/10.1007/s11726-009-0071-2

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  • DOI: https://doi.org/10.1007/s11726-009-0071-2

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