Effect of acupuncture-moxibustion on TNF-α, sTNFR-I and sTNFR-II of rats with Crohn’s disease

摘要

目的

观察针灸对克罗恩病大鼠血清TNF-α、 sTNFR-I、 sTNFR-II 的影响。

方法

采用TNBS制备大鼠克罗恩病模型, 随机分为模型组、 隔药灸组、 电针组, 并与正常大鼠作对照, 采用HE染色观察结肠黏膜组织病理学变化, 应用ELISA方法检测血清TNF-α、 sTNFR-I、 sTNFR-II 的含量。

结果

与正常组比较, CD大鼠血清TNF-α显著升高、 sTNFR-I和sTNFR-II 无显著变化。 经隔药灸和电针治疗后血清TNF-α水平显著降低, sTNFR-I和sTNFR-II 无显著变化。 CD大鼠结肠组织的炎症病变和异常的组织结构在隔药灸、 电针治疗后得到明显改善。

结论

隔药灸与电针均能够显著降低血清TNF-α的含量, 该作用可能是隔药灸与电针治疗CD的作用机制之一。

Abstract

Objective

To observe the effect of acupuncture-moxibustion on TNF-α, sTNFR-I and sTNFR-II in rats with Crohn’s disease (CD).

Method

The models of CD rats induced by TNBS were randomized into model group, herbal cake-partitioned moxibustion group and electroacupuncture group as well as a normal control group. The histopathological changes of colon mucus membrane were observed with HE staining and the contents of TNF-α, sTNFR-I and sTNFR-II were detected with ELISA method.

Results

When compared with the normal group, the TNF-α level in CD rats was substantially elevated and the sTNFR-I and sTNFR-II showed no marked changes. After herbal cake-partitioned moxibustion and electroacupuncture treatment, the TNF-α level in CD rats was substantially reduced and sTNFR-I and sTNFR-II showed no marked changes. The inflammatory lesions and abnormal structures of CD rats were significantly improved after herbal cake-partitioned moxibustion and electric stimulation.

Conclusion

Herbal cake-partitioned moxibustion and electroacupuncture can both remarkably reduce the TNF-α level in blood serum, and this might be one of the action mechanisms in the treatment of CD.