观察针灸对克罗恩病大鼠血清TNF-α、 sTNFR-I、 sTNFR-II 的影响。
采用TNBS制备大鼠克罗恩病模型, 随机分为模型组、 隔药灸组、 电针组, 并与正常大鼠作对照, 采用HE染色观察结肠黏膜组织病理学变化, 应用ELISA方法检测血清TNF-α、 sTNFR-I、 sTNFR-II 的含量。
与正常组比较, CD大鼠血清TNF-α显著升高、 sTNFR-I和sTNFR-II 无显著变化。 经隔药灸和电针治疗后血清TNF-α水平显著降低, sTNFR-I和sTNFR-II 无显著变化。 CD大鼠结肠组织的炎症病变和异常的组织结构在隔药灸、 电针治疗后得到明显改善。
To observe the effect of acupuncture-moxibustion on TNF-α, sTNFR-I and sTNFR-II in rats with Crohn’s disease (CD).
The models of CD rats induced by TNBS were randomized into model group, herbal cake-partitioned moxibustion group and electroacupuncture group as well as a normal control group. The histopathological changes of colon mucus membrane were observed with HE staining and the contents of TNF-α, sTNFR-I and sTNFR-II were detected with ELISA method.
When compared with the normal group, the TNF-α level in CD rats was substantially elevated and the sTNFR-I and sTNFR-II showed no marked changes. After herbal cake-partitioned moxibustion and electroacupuncture treatment, the TNF-α level in CD rats was substantially reduced and sTNFR-I and sTNFR-II showed no marked changes. The inflammatory lesions and abnormal structures of CD rats were significantly improved after herbal cake-partitioned moxibustion and electric stimulation.
Herbal cake-partitioned moxibustion and electroacupuncture can both remarkably reduce the TNF-α level in blood serum, and this might be one of the action mechanisms in the treatment of CD.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price includes VAT (USA)
Tax calculation will be finalised during checkout.
WU Huan-gan, ZHANG Lin-shan. Clinical Study of Herbs-partition Moxibustion on Crohn’s Disease. Modern Rehabilitation, 2000, 4(3): 397.
Morris GP, Beck Pl, Herridge MS, et al. Hapten-induced Model of Chronic Inflammation and Ulceration in the Rat Colon. Gastroenterology,1989, 96(3): 95–811.
DENG Chang-sheng, XIA Bing. Inflammatory Bowel Diseases. Beijing: People’s Medical Publishing House, 1998: 123.
Colombel JF, Loftus EV Jr, Tremaine WJ, et al. The Safety Profile of Infliximab in Patients with Crohn’s Disease: the Mayo Clinic Experience in 500 Patients. Gastroenterology, 2004, 126(1):19–31.
Hanauer SB, Feagan BG, Lichtenstein GR, et al. Maintenance Infliximab for Crohn’s Disease: the ACCENT I Randomised Trial, Lancet, 2002, 359(9317):1541–1549.
Aliprantis AO, Diez-Roux G, Mulder LC, et al. Do Amacrophages Kill Though Apoptosis. Immunol Today, 1996, 17(12): 573–578.
Fund Item: National Natural Science Foundation of China (30271638)
Author: SHI Zheng (1967–), female, research fellow
About this article
Cite this article
Shi, Z., Ma, Xp., Wu, Hg. et al. Effect of acupuncture-moxibustion on TNF-α, sTNFR-I and sTNFR-II of rats with Crohn’s disease. J. Acupunct. Tuina. Sci. 7, 29–32 (2009). https://doi.org/10.1007/s11726-009-0029-4
- Crohn’s Disease
- Acupuncture-moxibustion Therapy
- Tumor Necrosis Factoralpha
- Receptors, Tumor Necrosis Factor, Type I
- Receptors, Tumor Necrosis Factor, Type II