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SDS-PAGE analysis of components in serum with anti-asthma activity derived from rats treated by acupuncture

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摘要

目的

探索针刺抗哮喘大鼠血清差异组份的研究方法。

方法

用蛋白质SDS-PAGE 技术, 对针刺抗哮喘血清进行组份差异分析。

结果

SDS-PAGE电泳显示了针刺与不针刺血清中差异蛋白的存在: 1)与正常对照组相比, ADX_Acup组血清组份 I和Asthma_Acup 组血清组份I, 均有一染色增高条带, 其分子量为62.1kDa; 2)与正常对照组相比, ADX_Acup组血清组份III有二条染色增高条带, 其分子量分别为36.1kDa、 12.3 kDa; 3)与正常对照组相比, ADX_Acup组血清组份III有一条染色降低条带, 其分子量为34.6 kDa。 提示针刺对血清蛋白具有上调和下调的双向调节作用。

结论

针刺抗哮喘大鼠血清中存在不同的差异组份。 针刺对血清蛋白具有上调和下调的双向调节作用。

Abstract

Objective

To explore a method of analyzing the components of anti-asthma activity in serum derived from rats treated with acupuncture.

Methods

SDS-PAGE was used to analyze the diversity of the components of serum derived from rats treated with or without acupuncture treatment.

Results

SDS-PAGE images displayed that there are one deeply dyed band, whose molecular weight is 62.1 kDa, in the component I from asthma acupuncture group and ADX-asthma acupuncture group, two deeply dyed bands, whose molecular weight are 36.1kDa and 12.3 kDa, respectively, in the component III of ADX-asthma acupuncture group, and one dyed band, whose molecular weight is 34.6 kDa, in the segment III of ADX-asthma acupuncture group.

Conclusion

Acupuncture treatment could produce different components in the serum of rats, and may have bidirectional regulation on protein components in serum.

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Authors and Affiliations

  1. Shanghai Research Institute of Acupuncture and Meridian, Shanghai, 200030, P. R. China

    Yu Wang  (王 宇), Yong-qing Yang  (杨永清), Jian-mei Cui  (崔建美) & Yan-yan Liu  (刘艳艳)

  2. Shanghai Medical Collage of Fudan University, Shanghai, 200032, P. R. China

    Shu-lan Ma  (马淑兰)

Authors
  1. Yu Wang  (王 宇)
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  2. Yong-qing Yang  (杨永清)
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  3. Shu-lan Ma  (马淑兰)
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  4. Jian-mei Cui  (崔建美)
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  5. Yan-yan Liu  (刘艳艳)
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Additional information

Fund Items: National Natural Science Funds (90409014, 30171177, 30701123); National Basic Research Program of China (973 Program, 2005CB523306-5); Shanghai Committee of Science and Technique (02DZ19150-5); The Key laboratory of acupuncture-immune effects of State Administration of Traditional Chinese Medicine; Shanghai Leading Academic Discipline Project (S30304)

Author: WANG Yu (1973–), male, assistant research fellow

Corresponding author: YANG Yong-qing (1964–), male, research fellow

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Wang, Y., Yang, Yq., Ma, Sl. et al. SDS-PAGE analysis of components in serum with anti-asthma activity derived from rats treated by acupuncture. J. Acupunct. Tuina. Sci. 7, 8–12 (2009). https://doi.org/10.1007/s11726-009-0008-9

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  • DOI: https://doi.org/10.1007/s11726-009-0008-9

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