Effects of electroacupuncture on the ability of learning and memory in rats with ischemia-reperfusion injury

摘要

目的

观察电针预处理和电针治疗对缺血再灌注大鼠学习记忆功能的影响, 为针灸参与中风后学习记忆功能障碍的早期防治和临床应用提供实验基础。

方法

将72只清洁级雄性SD大鼠随机分为假手术组、 模型组、 术前电针组和术后电针组, 通过观测各组大鼠避낵试验潜伏期和错误次数、 神经功能缺损评分、 患侧海马胆碱能受体结合量, 分析术前和术后电针对脑缺血再灌注大鼠学习记忆功能的影响。

结果

1)缺血后大鼠出现不同程度神经功能缺损体征。 与假手术组相比, 模型组神经功能缺损评分明显降低, 避暗试验潜伏期和错误次数明显增加。 mAchR的特异性结合量降低, 与假手术组差异有显著性(P<0.05)。 2)电针缺血大鼠可降低MCAO大鼠神经功能缺损积分, 与模型组相比, 术前电针组、 术后电针组避낵试验潜伏期和错误次数, 均有显著性差异(P<0.05)。 3)与模型组比较, 术前电针组mAchR结合量明显升高(P<0.05), 术后电针组mAchR结合量略有升高。

结论

术前和术后电针均可延长避暗试验潜伏期, 减少错误次数, 而术前电针预处理可明显升高脑缺血再灌注所致异常降低的mAchR结合量, 从而改善中枢胆碱能系统功能, 改善学习记忆。 提示电针预处理对预防缺血性脑血맜病所致的学习记忆功能缺损有重要应用价值。

Abstract

Objective

To observe the effects of pretreatment and treatment with electroacupuncture on the ability of learning and memory in rats with ischemia-reperfusion injury, and to provide the research basis for preventing and treating the disorder of learning and memory in patients with apoplectic sequelae.

Methods

Seventy-two SD rats, clean class, were randomly divided into sham operation group, model group, pretreatment group, and treatment group. The step-through latency and number of errors, nerve injury score, and the number of cholinoceptor in the affected hippocampus were recorded to analyze the effects of pretreatment and treatment with electroacupuncture on the ability of learning and memory in rats with ischemia-reperfusion injury.

Results

1) The rats with ischemia had various signs of nerve injury. Compared with that in the sham operation rats, the nerve injury score in the model rats was lower, the step-through latency and number of errors were increased, and the content of special mAchR was decreased (P<0.05); 2) Electroacupuncture could decrease the nerve injury score of the model rats. Compared with those in the model group, the step-through latency and number of errors all had significant difference in the pretreatment and treatment groups (P<0.05); 3) Compared with that in the model group, the content of mAchR was increased in the pretreatment group (P<0.05), and increased a little in the treatment group.

Conclusion

Pretreatment and treatment with electroacupuncture can prolong step-through latency, and induce number of errors. Electroacupuncture pretreatment can increase the content of mAchR induced by ischemia-reperfusion injury, to improve the ability of learning and memory. Electroacupuncture pretreatment plays an important role in preventing the disorder of learning and memory in patients with ischemic cerebrovascular disease.