摘要
目的
观察小鼠艾灸血清体外对EL-4 淋巴瘤细胞Fas、 bcl-2mRNA及蛋白表达的影响。
方法
采用小鼠艾灸血清培养EL-4 淋巴瘤细胞24h后, 应用原位杂交方法检测EL-4细胞Fas、 bcl-2mRNA的表达, 免疫细胞化学方法观察Fas、 bcl-2 蛋白表达。
结果
与正常小鼠血清比较, 艾灸血清培养EL-4 细胞后, 在bcl-2 mRNA及蛋白水平上, 阳性细胞百分率均明显降低(P<0.05), 在Fas mRNA及蛋白水平上, 阳性细胞百分率均明显升高 P<0.05 。
结论
艾灸血清能够下调EL-4 细胞bcl-2 mRNA及蛋白的表达, 上调Fas mRNA及蛋白的表达。
Abstract
Objective
To observe the influence of moxibustion serum of mice on the expression of Fas, bcl-2 mRNA and protein of EL-4 lymphoma cells in vitro.
Method
The EL-4 lymphoma cells were cultivated for 24 h in the moxibustion serum of mice. The expression of Fas and bcl-2 mRNA of EL-4 lymphoma cells were detected by in-situ hybridization method, and the protein expression of Fas and bcl-2 were observed by the immuocytochemistry method.
Results
The expression of bcl-2 mRNA and protein decreased, and the expression of Fas mRNA and protein increased significantly in EL-4 cells, which were cultivated in the moxibustion serum compared those cultivated in normal mice serum (P<0.05).
Conclusion
Moxibustion serum could down-regulate the bcl-2 mRNA and protein and up-regulate the Fas mRNA and protein of EL-4 cells.
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Authors and Affiliations
Additional information
Fund Items: The Key Laboratory of Acupuncture-immune Effects of State Administration of Traditional Chinese Medicine; Shanghai Leading Academic Discipline Project (S30304)
Author: MA Xiao-peng (1973–), female, associate researcher
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Ma, Xp., Zhao, Cy., Chen, Yf. et al. Influence of moxibustion serum on the expression of fas bcl-2 mRNA and Protein of EL-4 lymphoma cells. J. Acupunct. Tuina. Sci. 6, 331–333 (2008). https://doi.org/10.1007/s11726-008-0331-6
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DOI: https://doi.org/10.1007/s11726-008-0331-6