Effects of electroacupuncture on serum indexes of rats with hepatic fibrosis

摘要

目的

观察电针对肝硬化大鼠肝组织病理学及血清学指标的影响, 初步探讨电针抗大鼠肝硬化的作用及其机制。

方法

采用四氯化碳诱导大鼠肝硬化模型, 随机分为电针组、 秋水仙碱组和模型组, 并与正常大鼠作对照, 应用形态学、 放射免疫分析技术, 检测各组大鼠肝组织胶原增生情况及血清透明质酸(HA)、 层粘连蛋白(LN)、 III型前胶原 (PIIINP)、 IV型胶原(C-IV)的变化。

结果

与正常组比较, 模型组大鼠肝组织胶原增生, 血清HA、 LN、 PIIINP含量明显增加; 与模型组比较, 电针组大鼠肝组织胶原面积及血清 HA、 LN含量明显减少, 血清PIIINP、 C-IV虽有减少趋势但无统计学意义。

结论

电针对大鼠实验性肝硬化具有一定的防治作用, 其作用机制可能与电针能够保护肝细胞、 抑制细胞外基质的合成和促进胶原产物的代谢有关。

Abstract

Objective

To observe the effects of electroacupuncture on the histopathological changes and serum indexes of rats’ liver, and to investigate the mechanism of electroacupuncture in treating hepatic fibrosis of rats.

Methods

The rat model of hepatic fibrosis was induced with carbon tetrachloride. Then, the rats were divided into electroacupuncture group, medicine group, and model group. The collagen of liver, and serum hyaluronic acid (HA), laminin (LN), procollagen III (PIIINP), and collagen IV (C-IV) were detected with morphologic methods and radioimmunoassay.

Results

Compared with normal rats, the collagen was hyperplasia in the liver tissue, and the contents of the serum HA, LN, and PIIINP were higher in the model group. The collagen area of liver tissue, and the contents of the serum HA, and LN were lower in rats treated with electroacupuncture than model rats. The contents of serum of PIIINP, and C-IV changed little.

Conclusion

Electroacupuncture had some effects of prevention and treatment, and the mechanism may relate to the effects of electroacupuncture in protecting liver cells, inhibiting synthesis of extracellular matrix, and promoting the breakup of collagen.