Skip to main content
Log in

Molecular characterization and fermentative hydrogen production of a wild anaerobe in clostridium genus

  • Research Article
  • Published:
Frontiers of Energy and Power Engineering in China Aims and scope Submit manuscript

Abstract

Anaerobic process of biohydrogen production is developed in this paper. The isolation and identification of high efficient biohydrogen production anaerobic bacteria are the important foundations for the fermented biohydrogen production process by anaerobic digesting organic wastewater. Taking the physiological and biochemical traits, the morphological characteristics and 16S rDNA sequence into consideration, the isolate Rennanqilyf33 is a new species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Levin D B P, Love M L. Biohydrogen production: Prospects and limitations to practical application. Int J Hydrogen Energy, 2004, 29: 173–185

    Article  Google Scholar 

  2. Hawkes R, Dionsdale D L, Hawkes I, et al. Sustainable fermentative hydrogen production: Challenges for process optimization. Int J Hydrogen Energy, 2002, 27: 1 339–1 347

    Google Scholar 

  3. Ren N Q. Bio-producing H2 principles and controlling strategy for organic wastewater treatment. Dissertation for the Doctoral Degree. Harbin: Harbin University of Architecture and Engineering, 1993 (in Chinese)

    Google Scholar 

  4. Li J Z. The bio-producing hydrogen technology of organic wastewater fermentation. Dissertation for the Doctoral Degree. Harbin: Harbin University of Architecture and Engineering, 1999 (in Chinese)

    Google Scholar 

  5. Qin Z. Controlling strategy of fermentation types and bioaugmentation in bio-hydrogen production reactor. Dissertation for the Doctoral Degree. Harbin: Harbin Institute of Technology, 2003 (in Chinese)

    Google Scholar 

  6. Li Y F, Ren N Q, Li J Z. The isolation and characterization of several strains of hydrogen-producing in biohydrogen production reactor. In: He Yanling, Gu Zhaolin, Chen Jierong, eds. Proceedings of the 9th Mainland—Taiwan Environmental Protection Academic Conference. Xi’an: Xi’an Communications University, 2004, 834–837 (in Chinese)

    Google Scholar 

  7. Tanisho S, Ishiwata Y. Continuous hydrogen production from molasses by the bacterium Enterobacter aerogenes. Int J Hydrogen Energy, 1994, 19(10): 807–812

    Article  Google Scholar 

  8. Tanisho S, Ishiwata Y. Continuous hydrogen production from molasses by fermentation using urethane foam as a support of flocks. Int J Hydrogen Energy, 1995, 20(7): 541–545

    Article  Google Scholar 

  9. Yokoi H, Ohkawara T, Hirose J, et al. Characteristics of hydrogen production by aciduric Enterobacter aerogenes strain HO-39. J Ferment Bioeng, 1995, 80(6): 571–574

    Article  Google Scholar 

  10. Yokoi H, Tokushige T, Hirose J, et al. Hydrogen production by immobilized cells of aciduric Enterobacter aerogenes strain HO-39. J Ferment Bioeng, 1997, 83(5): 481–484

    Article  Google Scholar 

  11. Rachman M A, Nakashimada Y, Eahizono T, et al. Hydrogen production with high yield and high evolution rate by self-flocculated cells of Enterobacter aerogenes in a packed-bed reactor. Appl Microbiol Biotechnol, 1998, 49: 450–454

    Article  Google Scholar 

  12. Yokoi H, Maeda Y, Hirose J. H2 Production by immobilization cells of Clostridium butyricum on porous glass beads. Biotechnology Techniques, 1997, 11(6): 431–433

    Article  Google Scholar 

  13. Okamoto M, Mlyahara T, Mizuno O, et al. Biological hydrogen potential of materials characteristic of the organic municipal solid wastes. Wat Sci Tech, 2000, 41(3): 25–32

    Google Scholar 

  14. Taguchi F, Mizukami N, Yamada K, et al. Hydrogen production from continuous fermentation of xylose during growth of Colstridium sp. No. 2. Can J Microbiol, 1995, 41: 536–540

    Article  Google Scholar 

  15. Kumar N, Das D. Biological hydrogen production in a packed bed reactor using agroresidues as solid matrices. In: Proceedings of the 13th World Hydrogen Energy Conference. Beijing: Science Press, 2000, 364–369

    Google Scholar 

  16. Oh Y K, Seol E H, Kim J R, at al. Fermentative biohydrogen production by a new chemoheterotrotrophic bacterium Citrobacter sp. Y 19. Int J Hydrogen Energy, 2003, 28: 1 353–1 359

    Google Scholar 

  17. Li B K. The principles of biohydrogen production by heavy organic wastewater-mechanism and ability of hydrogenproducing bacterium. Dissertation for the Doctoral Degree. Harbin: Harbin University of Architecture and Engineering, 1995 (in Chinese)

    Google Scholar 

  18. Lin M. Hydrogen-producing mechanism and ecological law of a new species for high efficiency hydrogen production by fermentation. Dissertation for the Doctoral Degree. Harbin: Harbin Institute of Technology, 2002 (in Chinese)

    Google Scholar 

  19. Wang Y. Controlling optimal fermentation type of biohydrogen production and high efficient hydrogen production bacterium. Dissertation for the Doctoral Degree. Harbin: Harbin Institute of Technology, 2002 (in Chinese)

    Google Scholar 

  20. Li Y F, Ren N Q, Chen Y, et al. Anaerobic operation of fermentative hydrogen production bacteria. Journal of Harbin Inst of Technology, 2004, 36(12): 1 589–1 592 (in Chinese)

    Google Scholar 

  21. Zhang R, Li Y F, Li J Z, et al. The isolation and evaluation of Lactobacillales on biohydrogen production by molasses. China Beet & Sugar, 2004, 1: 8–11 (in Chinese)

    Google Scholar 

  22. Holt J G, Krieg N R, Sneath P H A, et al. Bergey’s Manual of Determinative Bacteriology. 9th ed. Baltimore, Md: Williams & Wilkins, 1994

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Li Yongfeng or Ren Nanqi.

Additional information

__________

Translated from Acta Energlae Solaris Sinica, 2006, 27(2): 208–211 [译自: 太阳能学报]

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, Y., Ren, N., Yang, C. et al. Molecular characterization and fermentative hydrogen production of a wild anaerobe in clostridium genus. Front. Energy Power Eng. China 1, 403–407 (2007). https://doi.org/10.1007/s11708-007-0058-2

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11708-007-0058-2

Keywords

Navigation