Abstract
In the practices of cryobiology, selection of an optimum freeze/thawing program and an idealistic cryoprotective agent often requires rather tedious, time consuming and repetitive tests. Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program. Prototype devices were fabricated and corresponding experimental tests were performed. It was shown that microflow-channel chip could not offer a high quality solution distribution. As an alternative, the spot-dropping chip proved to be an excellent way to load the sample quickly and reliably. Infrared thermal mapping on such a chip showed that it had a rather uniform heat transfer boundary. Applying the spot-dropping chip combined with the thermoelectric cooling device, the final output of cryopreservation of multiple samples was tested, and the optimal freeze/thawing program as well as the potentially best concentration of the cryoprotective agent was found by analyzing the results. Further, application of this technique to measure the thermo-physical properties of the cryo-protective agent was also investigated. The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.
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References
Wesley S J, Walters C, Berjak P, et al. The influence of water content, cooling and warming rate upon survival of embryonic axes of Poncirus trifoliate. Cryoletters, 2004, 25(2): 129–138
Thirumala S, Ferrer M S, Jarrah A A, et al. Cryopreservation of canine spermatozoa: theoretical prediction of optimal cooling rates in the presence and absence of cryoprotective agents. Cryobiology, 2003, 47(2): 109–124
Karlsson J O M, Toner M. Long-term storage of tissues by cryopreservation: Critical issues. Biomaterials, 1996, 17(3): 243–256
Pegg D E. The history and principles of cryopreservation. Seminars in Reproductive Medicine, 2002, 20(1): 5–13
Wisniewski R. Large-scale cryopreservation of cells, cell components, and biological solutions. Biopharm—the Applied Technologies of Biopharmaceutical Development, 1998, 11(9): 42–50
Hua T C, Ren H S. Cryo-Biomedical Technology, Beijing: Science Press, 1994 (in Chinese)
Labbe C, Crowe L M, Crowe J H. Stability of the lipid component of trout sperm plasma membrane during freeze-thawing. Cryobiology, 1997, 34: 176–182
Byrd W. Cryopreservation, thawing, and transfer of human embryos. Seminars in Reproductive Medicine, 2002, 20(1): 37–43
Mazur P. Kinetics of water loss from cells at subzero temperatures and the likelihood of intracellular freezing. J Gen Physiol, 1963, 47: 347–369
Elford B C, Walter C A. Effect of electrolyte composition and function of smooth muscle cooled to −79°C in unfrozen media. Cryobiology, 1972, 9: 82–100
Smith A U. Biological Effect of Freezing and Supercooling, London: Edward Arnold, 1961
Liu J G, Liu Z B. Cryo-Medicine, Beijing: People’s Health Press, 1993 (in Chinese)
Liu J, Yu L N. Biochip testing device for quickly selecting optimum parameters for cryopreserving biological samples. China Patent CN200410037318.3, 2004
Dertinger S K W, Chiu D T, Jeon N L, et al. Generation of gradients having complex shapes using microfluidic networks. Anal Chem, 2001, 73: 1240–1246
Liu J, Zhou Y X. Freezing curve-based monitoring to quickly evaluate the viability of biological materials subject to freezing or thermal injury. Anal Bioanal Chem, 2003, 377: 173–181
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Translated from Cryogenics, 2006, (1): 21–26 [译自: 低温工程]
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Yu, L., Liu, J., Zhou, Y. et al. Biological chip technology to quickly batch select optimum cryopreservation procedure. Front. Energy Power Eng. China 1, 316–321 (2007). https://doi.org/10.1007/s11708-007-0046-2
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DOI: https://doi.org/10.1007/s11708-007-0046-2