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The comparison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty

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Abstract

Urethral strictures were common disease caused by over-expression of extracellular matrix from fibroblast. In this study, we compare two nanoyarn scaffolds for improving fibroblasts infiltration without inhibition the over-expression of extracellular matrix. Collagen/poly(L-lactide-co-caprolactone) (Col/P(LLA-CL)) nanoyarn scaffolds were prepared by conjugated electrospinning and dynamic liquid electrospinning, respectively. In addition, co-axial electrospinning technique was combined with the nanoyarn fabrication process to produce nanoyarn scaffolds loading Wnt signaling pathway inhibitor. The mechanical properties of the scaffolds were examined and morphology was observed by SEM. Cell morphology, proliferation and infiltration on the scaffolds were investigated by SEM, MTT assay and H&E staining, respectively. The release profiles of different scaffolds were determined using HPLC. The results indicated that cells showed an organized morphology along the nanoyarns and considerable infiltration into the nanoyarn scaffolds prepared by dynamic liquid electrospinning (DLY). It was also observed that the DLY significantly facilitate cell proliferation. The D-DLY could facilitate the infiltration of the fibroblasts and could be a promising scaffold for the treatment of urethra stricture while it may inhibit the collagen production.

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Authors and Affiliations

  1. State Key Lab for Modification of Chemical Fibers & Polymer Materials, College of Chemistry & Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China

    Xuran Guo, Mohamed El-Aassar & Xiumei Mo

  2. Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, Shanghai, 200030, China

    Kaile Zhang & Qiang Fu

  3. Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, 21934, Egypt

    Mohamed El-Aassar

  4. Shanghai Aquatic Product Research Institute, Shanghai, 200030, China

    Nanping Wang

  5. Department of Chemistry, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Hany El-Hamshary & Mohamed El-Newehy

  6. Department of Chemistry, Faculty of Science, Tanta University, Tanta, 31527, Egypt

    Hany El-Hamshary & Mohamed El-Newehy

  7. Shandong International Biotechnology Park Development Co., Ltd., Shanghai, 200030, China

    Xiumei Mo

Authors
  1. Xuran Guo
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  2. Kaile Zhang
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  3. Mohamed El-Aassar
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  4. Nanping Wang
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  6. Mohamed El-Newehy
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  7. Qiang Fu
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  8. Xiumei Mo
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Correspondence to Qiang Fu or Xiumei Mo.

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Guo, X., Zhang, K., El-Aassar, M. et al. The comparison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty. Front. Mater. Sci. 10, 346–357 (2016). https://doi.org/10.1007/s11706-016-0359-3

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  • DOI: https://doi.org/10.1007/s11706-016-0359-3

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