Skip to main content
SpringerLink
Log in

Synthesis, characterization and biological evaluation of poly [LA-co-(Glc-alt-Lys)] for nerve regeneration scaffold

  • Research Article
  • Published:
Frontiers of Materials Science Aims and scope Submit manuscript

Abstract

A novel nerve repairing material poly [LA-co-(Glc-alt-Lys)] (PLGL) was synthesized. The viability and growth of Schwann cells (SCs) co-cultured with poly (D, Llactic acid) (PDLLA) films (control group) and PLGL films were evaluated by MTTassay and SEM observation. Then, contact angle measurement, histological assessment and enzyme-linked immunosorbent assay (ELISA) testing on inflammatory-related cytokines such as IL-10 and TGF-β1 were performed. The results showed that, compared with PDLLA, PLGL films possesses better hydrophilicity, biocompatibility, degradation property and less inflammatory reaction. The present study indicated that PLGL scaffolds would meet the requirements of artificial nerve scaffold and have a potential application in the fields of nerve regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Afshari F T, Kwok J C, White L, et al. Schwann cell migration is integrin-dependent and inhibited by astrocyte-produced aggrecan. Glia, 2010, 58(7): 857–869

    Google Scholar 

  2. Zhu X, Yao L, Yang X, et al. Spatiotemporal expression of KHSRP modulates Schwann cells and neuronal differentiation after sciatic nerve injury. The International Journal of Biochemistry & Cell Biology, 2014, 48(48): 1–10

    Article  Google Scholar 

  3. Wan Z-T, Yin Y-X, Wang Y-H, et al. Research on the release of nerve growth factor from PNGF nerve repair material in vitro. Journal of Wuhan University of Technology, 2010, 32(12): 19–21

    Google Scholar 

  4. Phillips J B, King V R, Ward Z, et al. Fluid shear in viscous fibronectin gels allows aggregation of fibrous materials for CNS tissue engineering. Biomaterials, 2004, 25(14): 2769–2779

    Article  Google Scholar 

  5. Barnett S C, Roskams A J. Olfactory ensheathing cells: isolation and culture from the neonatal olfactory bulb. Methods in Molecular Biology, 2008, 438: 85–94

    Article  Google Scholar 

  6. Karimi M, Biazar E, Keshel S H, et al. Rat sciatic nerve reconstruction across a 30 mm defect bridged by an oriented porous PHBV tube with Schwann cell as artificial nerve graft. ASAIO Journal, 2014, 60(2): 224–233

    Article  Google Scholar 

  7. Martin I, Nguyen T D, Krell V, et al. Generation of Schwann cellderived multipotent neurospheres isolated from intact sciatic nerve. Stem Cell Reviews, 2012, 8(4): 1178–1187

    Article  Google Scholar 

  8. Sedaghati T, Jell G, Seifalian A. Investigation of Schwann cell behaviour on RGD-functionalised bioabsorbable nanocomposite for peripheral nerve regeneration. New Biotechnology, 2014, 31(3): 203–213

    Article  Google Scholar 

  9. Tao Y. Isolation and culture of Schwann cells. Methods in Molecular Biology, 2013, 1018: 93–104

    Article  Google Scholar 

  10. Spiller K L, Anfang R R, Spiller K J, et al. The role of macrophage phenotype in vascularization of tissue engineering scaffolds. Biomaterials, 2014, 35(15): 4477–4488

    Article  Google Scholar 

  11. McAlvin J B, Padera R F, Shankarappa S A, et al. Multivesicular liposomal bupivacaine at the sciatic nerve. Biomaterials, 2014, 35(15): 4557–4564

    Article  Google Scholar 

  12. Bailey A, van Hateren A, Elliott T, et al. Two polymorphisms facilitate differences in plasticity between two chicken major histocompatibility complex class I proteins. PLoS One, 2014, doi: 10.1371/journal.pone.0089657

    Google Scholar 

  13. Ni B, Jia Z, Wu Y. Preparation of polypeptides comprising multiple TAA peptides. Methods in Molecular Biology, 2014, 1139: 357–366

    Article  Google Scholar 

  14. Atkins S, Loescher A R, Boissonade F M, et al. Interleukin-10 reduces scarring and enhances regeneration at a site of sciatic nerve repair. Journal of the Peripheral Nervous System, 2007, 12(4): 269–276

    Article  Google Scholar 

  15. Taskinen H S, Olsson T, Bucht A, et al. Peripheral nerve injury induces endoneurial expression of IFN-γ, IL-10 and TNF-α mRNA. Journal of Neuroimmunology, 2000, 102(1): 17–25

    Article  Google Scholar 

  16. Zhou L, Lopes J E, Chong M M, et al. TGF-β-induced Foxp3 inhibits T(H)17 cell differentiation by antagonizing RORgammat function. Nature, 2008, 453(7192): 236–240

    Article  Google Scholar 

  17. Norden DM, Fenn AM, Dugan A, et al. TGFβ produced by IL-10 redirected astrocytes attenuates microglial activation. Glia, 2014, doi: 10.1002/glia.22647

    Google Scholar 

  18. Veldhoen M, Uyttenhove C, van Snick J, et al. Transforming growth factor-β ‘reprograms’ the differentiation of T helper 2 cells and promotes an interleukin 9-producing subset. Nature Immunology, 2008, 9(12): 1341–1346

    Article  Google Scholar 

  19. Dardalhon V, Awasthi A, Kwon H, et al. IL-4 inhibits TGF-β-induced Foxp3+ T cells and, together with TGF-β, generates IL-9 + IL-10+ Foxp3(−) effector T cells. Nature Immunology, 2008, 9(12): 1347–1355

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biomedical Materials and Engineering Research Center, Wuhan University of Technology, Wuhan, 430070, China

    Yi-Xia Yin, Ji-Ling Yi, Li-Juan Xie, Qiong-Jiao Yan, Hong-Lian Dai & Shi-Pu Li

Authors
  1. Yi-Xia Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ji-Ling Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Li-Juan Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qiong-Jiao Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hong-Lian Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shi-Pu Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shi-Pu Li.

Additional information

Y.X.Y. and J.L.Y. contributed to this paper equally.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yin, YX., Yi, JL., Xie, LJ. et al. Synthesis, characterization and biological evaluation of poly [LA-co-(Glc-alt-Lys)] for nerve regeneration scaffold. Front. Mater. Sci. 8, 95–101 (2014). https://doi.org/10.1007/s11706-014-0239-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11706-014-0239-7

Keywords

Search

Navigation