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Effect of incorporating Elaeagnus angustifolia extract in PCL-PEG-PCL nanofibers for bone tissue engineering

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Abstract

Plants have been used for medicinal purposes for thousands of years but they are still finding new uses in modern times. For example, Elaeagnus angustifolia (EA) is a medicinal herb with antinociceptive, anti-inflammatory, antibacterial and antioxidant properties and it is widely used in the treatment of rheumatoid arthritis and osteoarthritis. EA extract was loaded onto poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) (PCL-PEG-PCL/EA) nanofibers and their potential applications for bone tissue engineering were studied. The morphology and chemical properties of the fibers were evaluated using Fourier transform infrared spectroscopy, field emission scanning electron microscopy, contact angle measurements and mechanical tests. All the samples had bead-free morphologies with average diameters ranging from 100 to 200 nm. The response of human cells to the PCL-PEG-PCL/EA nanofibers was evaluated using human dental pulp stem cells (hDPSCs). The hDPSCs had better adhesion and proliferation capacity on the EA loaded nanofibers than on the pristine PCL-PEG-PCL nanofibers. An alizarin red S assay and the alkaline phosphatase activity confirmed that the nanofibrous scaffolds induced osteoblastic performance in the hDPSCs. The quantitative real time polymerase chain reaction results confirmed that the EA loaded nanofibrous scaffolds had significantly upregulated gene expression correlating to osteogenic differentiation. These results suggest that PCL-PEG-PCL/EA nanofibers might have potential applications for bone tissue engineering.

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Acknowledgements

The authors were financially supported by a grant (No. 94/104) from the Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

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Authors and Affiliations

  1. Department of Chemistry, University of Zanjan, Zanjan, Iran

    Vahideh R. Hokmabad & Ali Ramazani

  2. Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran

    Soodabeh Davaran & Roya Salehi

  3. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Marziyeh Aghazadeh & Effat Alizadeh

  4. Oral Medicine Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran

    Marziyeh Aghazadeh

  5. Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran

    Effat Alizadeh

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  1. Vahideh R. Hokmabad
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  3. Marziyeh Aghazadeh
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  4. Effat Alizadeh
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  5. Roya Salehi
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Correspondence to Roya Salehi or Ali Ramazani.

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Hokmabad, V.R., Davaran, S., Aghazadeh, M. et al. Effect of incorporating Elaeagnus angustifolia extract in PCL-PEG-PCL nanofibers for bone tissue engineering. Front. Chem. Sci. Eng. 13, 108–119 (2019). https://doi.org/10.1007/s11705-018-1742-7

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