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Study on microbial protein and the mechanism of solid-state fermentation with periodical dynamic changes of air

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Abstract

The effect of different extraction and purification conditions on the microbial protein obtained from solid-state fermentation (SSF) and the effect of periodical dynamic changes of air on protein have been studied. The mechanism of solid-state fermentation with periodical dynamic changes of air is also discussed. Compared with static solid-state fermentation, periodical dynamic changes of air afford a higher protein mass; from 1 g of the fermentation microbe, 5.3 mg of the intracellular protein (an increase of 34.63%) and 9.09 mg of the extracellular protein (an increase of 17.8%) were obtained on the sixth day of fermentation. The filter paper activity and carboxymethyl cellulose activity (FPA and CMCase) of the extracellular protein are 1.739 μmol/s and 109.592 μmol/s, respectively, which represent increases of 60.1% and 21.2% over the corresponding values for static solid-state fermentation. The FPA and CMCase of the intracellular protein are 0.245 μmol/s and 6.392 μmol/s, respectively, which represent decreases of 22.2% and 38.7% over the corresponding values for static solid-state fermentation. The enzyme activity of the microbial extracellular protein in solid-state fermentation with periodical dynamic changes of air on the fifth day is nearly equal to that on the sixth day without periodical dynamic changes of air, so the period of fermentation can be shortened. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) experiments suggest that pulsating air pressure stimulation leads to a decrease in the amount of protein component with molecule mass of about 80400Da, and an increase in the amount of protein component with molecule mass of about 28520Da.

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Authors and Affiliations

  1. Key State Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, China

    Fu Xiaoguo, Chen Hongzhang & Li Hongqiang

  2. Key Laboratory of Science and Technology of Controllable Chemical Reactions, Ministry of Education, Beijing University of Chemical Technology, Beijing, 100029, China

    Ma Runyu

Authors
  1. Fu Xiaoguo
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  2. Chen Hongzhang
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  3. Li Hongqiang
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  4. Ma Runyu
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Corresponding author

Correspondence to Chen Hongzhang.

Additional information

Translated from Journal of Beijing University of Chemical Technology, 2006, 33(3): 42–46 [译自: 北京化工大学学报]

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Fu, X., Chen, H., Li, H. et al. Study on microbial protein and the mechanism of solid-state fermentation with periodical dynamic changes of air. Front. Chem. Eng. China 1, 113–117 (2007). https://doi.org/10.1007/s11705-007-0021-9

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  • DOI: https://doi.org/10.1007/s11705-007-0021-9

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