iRNA-PseTNC: identification of RNA 5-methylcytosine sites using hybrid vector space of pseudo nucleotide composition


RNA 5-methylcytosine (m5C) sites perform a major role in numerous biological processes and commonly reported in both DNA and RNA cellular. The enzymatic mechanism and biological functions of m5C sites in DNA remain the focusing area of researchers for last few decades. Likewise, the investigators also targeted m5C sites in RNA due to its cellular functions, positioning and formation mechanism. Currently, several rudimentary roles of the m5C in RNA have been explored, but a lot of improvements are still under consideration. Initially, the identification of RNA methylcytosine sites was carried out via experimental methods, which were very hard, erroneous and time consuming owing to partial availability of recognized structures. Looking at the significance of m5C role in RNA, scientists have diverted their attention from structure to sequence-based prediction. In this regards, an intelligent computational model is proposed in order to identify m5C sites in RNA with high precision. Three RNA sequences formulation methods namely: pseudo dinucleotide composition,pseudo trinucleotide composition and pseudo tetra nucleotide composition are applied to extract variant and high profound numerical features. In a sequel, the vector spaces are fused to build a hybrid space in order to compensate the weakness of each other. Various learning hypotheses are examined to select the best operational engine, which can truly identify the pattern of the target class. The strength and generalization of the proposed model are measured using two different cross validation tests. The reported outcomes reveal that the proposed model achieved 3% better accuracy than that of the highest present approach in the literature so far.

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We thank to the anonymous reviewers for their careful reading of our manuscript and their useful comments and suggestions.

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Corresponding author

Correspondence to Maqsood Hayat.

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Shahid Akbar received his Bachelor degree in Computer Science & Information Technology from Islamic University of Technology, Bangladesh in 2011. He received his MS degree in Computer Science from Abdul Wali Khan University (AWKU), Pakistan in 2015. He is currently pursuing his PhD in Computer Science from Abdul Wali Khan University (AWKU), Pakistan. His research interests include bioinformatics, pattern recognition and machine learning.

Maqsood Hayat received his MCS degree from Gomal University, Pakistan in 2004 and his MS degree in Software & System Engineering from Mohammad Ali Jinnah University (MAJU), Pakistan in 2009. He received his PhD degree from Department of Computer & Information Sciences, Pakistan Institute of Engineering & Applied Sciences, Pakistan. He is working as an assistant professor since august 2012. His main research includes machine learning, pattern recognition, evolutionary computing and its application in bioinformatics.

Muhammad Iqbal received his Bachelor degree in Computer Science from Islamia College University, Pakistan in 2012. He is currently pursuing his MS degree in Computer Science from Abdul Wali Khan University (AWKUM), Pakistan. His research interests include bioinformatics, pattern recognition and machine learning.

Muhammad Tahir received his PhD degree in Computer Science from Abdul Wali Khan University (AWKUM), Pakistan in 2017. He is working as a lecturer since August 2010. His main research includes pattern recognition, bioinformatics and machine learning.

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Akbar, S., Hayat, M., Iqbal, M. et al. iRNA-PseTNC: identification of RNA 5-methylcytosine sites using hybrid vector space of pseudo nucleotide composition. Front. Comput. Sci. 14, 451–460 (2020).

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  • methylcytosine sites
  • PseTNC
  • PseTetraNC
  • hybrid features
  • SVM
  • cross validation test