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Study on separation and purification of oligomeric proanthocyanidin from Rhodiola rosea

Frontiers of Agriculture in China volume 5, pages 637–642 (2011)Cite this article

Abstract

The objective was to study the technology for the separation and purification of oligomeric proanthocyanidins from Rhodiola rosea and establish the best operating conditions. First, the oligomeric proanthocyanidins was extracted by ethyl acetate from rough-extracted R. rosea liquid using an optimized technique. Its purification was achieved by macroporous resins. Five kinds of macroporous adsorbent resins were compared for the adsorption and desorption performance of procyanidins, the concentration and pH value of both the extracted sample and the eluant were investigated. According to the results, the optimized conditions were as follows: four times of extraction at 25 min each time was effective, the best volume ratio was 1.5:1 (ethyl acetate: extracted solution); AB-8 resin was the best choice; the concentration of the extracted sample was 4.0 g/L, and the pH was 4.5; the ratio of the diameter to height of the chromatography column was 1:40 (cm); 50% ethanol was used as the eluant at pH 5; and finally, the purity of procyanidins reached 88. 3%.

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References

  • Bagchi D, Garg A, Krohn R L, Bagchi M, Bagchi D J, Balmoori J, Stohs S J (1998). Protective effects of grape seed proanthocyanidins and selected antioxidants against TPA-induced hepatic and brain lipid peroxidation and DNA fragmentation, and peritoneal macrophage activation in mice. Gen Pharmacol, 30(5): 771–776

    Article  PubMed  CAS  Google Scholar 

  • Bagchi D, Garg A, Krohn R L, Bagchi M, Tran M X, Stohs S J (1997). Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro. Res Commun Mol Pathol Pharmacol, 95(2): 179–189

    PubMed  CAS  Google Scholar 

  • Dong A W, Yu H Z, Feng Y Y (2010). Extraction and purification of procyanidins from the seeds of Parthenocissus tricuspidata. Food Science, 14(31): 88–92 (in Chinese)

    Google Scholar 

  • Eng E T, Ye J J, Williams D, Phung S, Moore R E, Young M K, Gruntmanis U, Braunstein G, Chen S (2003). Suppression of estrogen biosynthesis by procyanidin dimers in red wine and grape seeds. Cancer Res, 63(23): 8516–8522

    PubMed  CAS  Google Scholar 

  • Farhath K, Amarinder S B, Brahm S (2005). Rhodiola rosea:A Versatile Adaptogen. Comprehensive reviews in food science and food safety, 4: 55–62

    Article  Google Scholar 

  • Gad G Y, Grace M H, Cheng D M, Belolipov I V, Raskin I, Lila M A (2006). Comparative phytochemical characterization of three Rhodiola species. Phytochemistry, 67(21): 2380–2391

    Article  CAS  Google Scholar 

  • Han J, Li Y, Liu X P, Wang Y, Yao L B, Yu Q (2003). Inducing effect of proanthocyanidin from seed of Vitis vinif era on anoikis of breast cancer cells MCF-7. Chin Tradit Herbal Drugs, 34(8): 722–725 (in Chinese)

    CAS  Google Scholar 

  • Lu Y, Zhao W Z, Chang Z, Chen W X, Li L (2004). Procyanidins from grape seeds protect against phorbol ester-induced oxidative cellular and genotoxic damage. Acta Pharmacol Sin, 25(8): 1083–1089

    PubMed  CAS  Google Scholar 

  • Mei J L, Hu C Y, Tang N C (2010). Extraction and purification of procyanidine from sea buckthorn seed meal. China Oils and Fats, 7(35): 50–53 (in Chinese)

    Google Scholar 

  • Meng Y C, Zhao W, Guo X, Liu Y F, Feng L J (2007). Extraction and purification of proanthocyanidins from R. roaea. Agriculture Engineering Technology, (7): 48–51 (in Chinese)

  • Meng Y C, Zhao W, Li H L, Zhang H Y (2009). Primary study on the determination of procynidins content of Rhodiola roaea extract. Food Research and Development, 30(4): 133–136 (in Chinese)

    Google Scholar 

  • Panossian A, Wikman G, Sarris J (2010). Rosenroot (Rhodiola rosea): traditional use, chemical composition, pharmacology and clinical efficacy. Phytomedicine, 17(7): 481–493

    Article  PubMed  CAS  Google Scholar 

  • Petkov V D, Yonkov D, Mosharoff A (1986). Effects of alcohol aqueous extract from Rhodiola rosea roots on learning and memory. Acta Physiol Pharmacol Bulg, 12(1): 3–16

    CAS  Google Scholar 

  • Sano T, Oda E, Yamashita T, Naemura A, Ijiri Y, Yamamoto J (2005). Anti-thrombotic effect of proanthocyanidin, a purified ingredient of grape seed. Thromb Res, 115(1–2): 115–121

    Article  PubMed  CAS  Google Scholar 

  • Wang Q Y, Yu J Q, Yang W K (2009). Extraction and purification of proanthocyanidins from pine bark. Journal of Mountain Agriculture and Biology, 28(5): 436–440 (in Chinese)

    Google Scholar 

  • Wen G, Li L Q, Sui X (2010). Extraction of oligomeric proanthocyanidins from grape seeds. China Brewing, 1: 111–113 (in Chinese)

    Google Scholar 

  • Wu W C, Wang F L, Ding Z G (1994). Introduction and cultivation techniques of Rhodiola rosea. Primary Journal of Chinese Materia Medica, 2(2): 16–18 (in Chinese)

    Google Scholar 

  • Zhao W, Wang T X, Qi X J (2006). The anti-fatigue effect of Rhodiola rosea extract in mice. Acta Nutrimenta Sinica, 28(2): 180–181 (in Chinese)

    Google Scholar 

  • Zhou W J, Sun Z D, Xie B J (2009). Technology optimization for extracting procyanidins from litchi pericarp. Transactions of the CSAE, 25(Suppl 1): 175–179 (in Chinese)

    Google Scholar 

Download references

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Authors and Affiliations

  1. College of Food Science and Technology, Hebei Agriculture University, Engineering Technology Research Center for Agricultural Product Processing of Hebei, Baoding, 071001, China

    Zhiping Yin, Hongyu Chen, Sisi Wang & Wen Zhao

  2. College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, 300134, China

    Boyang Zhang

Authors
  1. Zhiping Yin
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  2. Boyang Zhang
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  3. Hongyu Chen
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  4. Sisi Wang
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  5. Wen Zhao
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Correspondence to Wen Zhao.

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Yin, Z., Zhang, B., Chen, H. et al. Study on separation and purification of oligomeric proanthocyanidin from Rhodiola rosea . Front. Agric. China 5, 637–642 (2011). https://doi.org/10.1007/s11703-011-1137-8

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  • DOI: https://doi.org/10.1007/s11703-011-1137-8

Keywords

  • Rhodiola rosea
  • oligomeric proanthocyanidins
  • extraction
  • macroporous resins
  • separation and purification