Skip to main content
Log in

Observation of the infection process of Metarhizium anisopliae on the cuticle of Anoplophora glabripennis larvae with scanning electron microscopy

  • Research Article
  • Published:
Frontiers of Agriculture in China

Abstract

The invasion behavior and infection process of Metarhizium anisopliae on different cuticle areas of Anoplophora glabripennis larvae were observed by scanning electron microscopy. The results indicated that M. anisopliae infected A. glabripennis larvae mainly through the intersegmental membrane where more attaching conidia, faster germination, and higher germination and penetration rates were observed. The secondary invasion area was around the valve. The two areas were vulnerable to infection from M. anisopliae. Twelve hours after vaccination, conidia germinated and bud-shaped protrusions formed on the cuticle of A. glabripennis larvae, then germ tubes and various attaching structures were produced. Conidia penetrated the integument into the hemocoele of A. glabripennis larvae 36–48 h later.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Fan M Z, Guo C, Yan X H (1987). Test of five entomogenous fungi Saperda populnea L.. Acta Mycologica Sinica, 6(2): 97–102 (in Chinese)

    Google Scholar 

  • Fan M Z, Li Z Z (1994). Impact of nutrients and culture conditions on Appressorium formation of entomogenous fungi. Journal of Anhui Agricultural University, 21(2): 123–130 (in Chinese)

    Google Scholar 

  • He X Y, Chen S L, Huang J S (2005). Preliminary screening of virulent strains of Metarhizium anisopliae against Monochamus alternatus. Acta Entomologica Sinica, 48(6): 975–981 (in Chinese)

    Google Scholar 

  • He X Y, Chen S L, Yang X (2007). The investigation of Metarhizium anisopliae in forest soil in Fujian and Jiangxi provinces and pathogenicity against Monochamus alternatus. Acta Mycologica Sinica, 26(2): 289–294 (in Chinese)

    CAS  Google Scholar 

  • Tong S L, Gong J, Gou Y J (1991). Study of Control of Saperda Populnea by Applying Metarhizium anisopliae. Study and Application of Entomogenous Fungi in China, III. Beijing: China Agriculture Press, 155–157 (in Chinese)

    Google Scholar 

  • Wang H C, You M S (1999a). Mechanism of invasion by Metarhizium anisopliae against insect. Microbiology, 26(1): 71–73 (in Chinese)

    Google Scholar 

  • Wang H C, You M S (1999b). Advances in conidia attachment by Metarhizium anisopliae on the host. Ento-mological Knowledge, 36(3): 189–192 (in Chinese)

    Google Scholar 

  • Wang S B, Huang Y P, Zhang X T (2004). Screening and biological control of high virulent strains against Monochamus alternatus adults. Forest Pest and Disease, 23(6): 13–16 (in Chinese)

    Google Scholar 

  • Wang Z G (2004). Study on the occurrence dynamics of Anoplophora glabripennis (Coleoptera:Cerambycidae) and its control measures. Dissertation for the Doctoral Degree. Haerbing: Northeast Forestry University

    Google Scholar 

  • Xia C R, Ding D G, Liu Y P (2005). Field trials against Spondylis buprestoides through combined use of non-woven fabric bands impregnated with Metarhizium anisopliae and an attractant. Journal of Anhui Agricultural University, 32(4): 419–422 (in Chinese)

    Google Scholar 

  • Xu J Z (2002). Study on application of entomogenous fungi against Monochamus alternatus and Anoplophora glabripennis. Dissertation for the Masterl Degree. Hefei: Anhui Agricultural University

    Google Scholar 

  • Zhang F R, Su H T, Ma P (1997). Scanning electron microscopic study on organs of Anoplophora glabripennis (Motschulsky). Journal of Inner Mongolia Institute of Agriculture & Animal Husbandry, 18(2): 55–59 (in Chinese)

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Dazhuang Huang.

About this article

Cite this article

Yan, H., Wang, D., Zhao, X. et al. Observation of the infection process of Metarhizium anisopliae on the cuticle of Anoplophora glabripennis larvae with scanning electron microscopy. Front. Agric. China 5, 662–665 (2011). https://doi.org/10.1007/s11703-011-1134-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11703-011-1134-y

Keywords

Navigation